Ivuka rya PCR
PCR reaction Urunigi rwa Polymerase）
Haraheze imyaka irenga 30 kuva havumbuwe urunigi rwa polymerase.Mu myaka irenga 30, nyuma yintiti nyinshi kwisi zikomeje kuzuza no kunoza, ikoranabuhanga rya PCR ryahindutse cyane kandi rikoreshwa cyane kandi ryingenzi ryubushakashatsi bwibanze mubuzima bwose.
TouchDown PCR, Real-Time PCR, Multi PCR, nibindi byatejwe imbere hashingiwe ku buryo bwagutse bwo gukoresha ikoranabuhanga gakondo rya PCR, kimwe na Digital PCR iherutse kuvuka (Digital PCR), byateje imbere cyane uburyo bw’ubushakashatsi bwa benshi mu bashakashatsi mu bya siyansi kandi byihutisha cyane iterambere ry’ubumenyi bw’ubuzima bwa none, cyane cyane ibinyabuzima bya molekuline, byagize uruhare runini mu kwiga ubuzima n’ubuzima bwa muntu muri rusange.
Inenge yubuhanga gakondo bwa PCR
Gutandukanya aside nucleic igoye kandigukuramo:
Technology Ubuhanga gakondo bwa PCR: birakenewe
★ PCR ikomoka ku ikoranabuhanga: irakenewe
Ingero za ADN na RNA: itandukaniro rinini, ibisabwa bigoye gukora
Dangers Ibyago byumubiri: reagent toxic yangiza umubiri
Ubuhanga gakondo bwa PCR hamwe nubuhanga bukomokaho bifite progaramu ya-nucleic aside itandukanya no kwezwa
Icyitegererezo cyibinyabuzima cyose kigomba kunyura murukurikirane rwibikorwa bigoye kandi birambiranye kugirango ubone urugero rwa acide nucleic yujuje ibisabwa na tekinoroji ya PCR.
Gutandukanya no gukuramo ADN na RNA byahoze ari umurimo wibanze abashakashatsi ba siyanse bakeneye bakeneye gusubiramo buri munsi.
Kubera itandukaniro rinini hagati yintangarugero, uburyo bwo gutandukanya no gukuramo ADN na RNA nabyo biratandukanye cyane.Aka kazi gasaba ubumenyi buhanitse bwa tekinike kubakoresha.Gutandukanya gakondo no kuvoma bisaba guhuza igihe kirekire na reagent ya chimique ifite ubumara bukabije.Bizatera ibyangiritse bidasubirwaho kumubiri wumukoresha, ndetse bizanangiza ibyangiritse mugihe cyibigeragezo.
Muri icyo gihe, kubafite umubare munini wintangarugero zo kwiga, gutandukana no gukuramo acide nucleic ni umurimo usaba akazi.
Nucleic aside yo kwigunga no gukuramo ibikoresho ku isoko ubu birakuze kandi hariho ibirango byinshi, ariko birasa.Yaba silika gel membrane inkingi ya centrifugal kit cyangwa ibikoresho bya magnetiki yamashanyarazi, bifata umwanya munini kandi birahenze.Usibye ikiguzi cyibikoresho, hari nibisabwa byihariye kubikoresho bya laboratoire.Ahantu hakorerwa imirimo ikoreshwa muburyo bwa magnetique ni uburyo busanzwe bunini bunini cyane bufite agaciro kanini, nigiciro kinini kuri laboratoire.
Muri make
Mbere yo gukora ubushakashatsi bwa PCR, kwitegura ingero byanze bikunze kandi burigihe kubabaza umutwe kubashakashatsi.Nigute wakemura iki kibazo kandi niba ubushakashatsi bwa PCR bushobora gukorwa hatabayeho gutandukanya no gukuramo acide nucleic yamye ari igitekerezo cya benshi mubashakashatsi ba siyanse n'abakozi ba laboratoire.
Umuti wa Foregene
Nyuma yimyaka myinshi yubushakashatsi bwimbitse kubijyanye na tekinoroji ya PCR nibikoresho bifitanye isano, Forgene yatsinze neza ibyuho byinshi kandi igera kuri PCR itaziguye kubwoko butandukanye bw'ingero zitandukanye zifite imbaraga zo guhangana n’imihindagurikire y'ikirere, bituma abashakashatsi bakuraho gutandukana bitoroshye kandi biteje akaga no gukuramo aside nucleique.Ibi bizagabanya cyane imbaraga za buriwese, byihutishe inzira yubushakashatsi, kandi bizigame ubushakashatsi bwa siyansi nigiciro cyo gupima.
Wibagirwe gusobanukirwa nubumenyi bwa DirectPCR
Ubwa mbere, tekinoroji ya DirectPCR nubuhanga bwa PCR butaziguye kubice bitandukanye byintangarugero.Muri ubu buryo bwa tekiniki, nta mpamvu yo gutandukanya no gukuramo aside nucleic, kandi icyitegererezo cya tissue gikoreshwa muburyo butaziguye, kandi intego ya gene yibanze yongeweho kugirango PCR ibe.
Icya kabiri, tekinoroji ya DirectPCR ntabwo ari tekinoroji gakondo ya ADN yerekana uburyo bwo kongera imbaraga, ahubwo ikubiyemo na RNA template revers transcript PCR.
Icya gatatu, tekinoroji ya DirectPCR ntabwo ikora gusa muburyo busanzwe bwa PCR ibyitegererezo byintangarugero, ahubwo inashyiramo reaction ya Real-Time qPCR, isaba sisitemu yogukora kugira ubushobozi bukomeye bwo kurwanya kwivanga kwa fluorescence no kurwanya inzitizi ya fluorescence.
Icya kane, ingero za tissue zigenewe tekinoroji ya DirectPCR zisaba gusa kurekura inyandikorugero ya acide nucleic kandi ntikureho proteyine, polysaccharide, ion yumunyu, nibindi bibangamira reaction ya PCR.Ibi bisaba aside nucleic aside polymerase na PCR Ivanga muri sisitemu yogukora kugirango igire anti-reversible nziza kandi ihindagurika, kandi irashobora kwemeza ibikorwa bya enzyme no kwigana neza mubihe bigoye.
Icya gatanu, ingero za tissue zigenewe ikoranabuhanga rya DirectPCR ntabwo zigeze zikoreshwa muburyo bwo gutunganya aside nucleique, kandi ingano yicyitegererezo ni nto cyane, bisaba kwiyumvamo cyane no kongera imbaraga za sisitemu yo kubyitwaramo.
Umwanzuro
Ikoranabuhanga rya DirectPCR nimwe mubintu byingenzi byiterambere byikoranabuhanga no guhanga udushya mumyaka 30 ishize kuva tekinoroji ya PCR ivuka.Wibagiwe afite kandi azakomeza kuba intangarugero no guhanga udushya twikoranabuhanga.
Ibyifuzo byo gukoresha tekinoroji ya DirectPCR ni ngari cyane.Gukomeza kunoza no guteza imbere iryo koranabuhanga rwose bizazana impinduka mbi mubikorwa byubushakashatsi nubugenzuzi.Iyi ni impinduramatwara ya PCR.
Igihe cyo kohereza: Gashyantare-21-2017
