Ikintu cyihariye kiranga PCR nigikorwa cyayo kinini cyo kongera imbaraga hamwe no kumva cyane.Kugirango tunoze imikorere ya PCR no kumenya neza, twiyemeje kunoza ubushobozi bwa PCR bwo kongera imbaraga no kumenya ibyiyumvo, ariko kubabara umutwe biri mubikorwa byubushakashatsi.Ibyiza bitari byiza bikunze kubaho, kandi umubare muto cyane wicyitegererezo cyanduzanya cyangwa ibicuruzwa bya PCR bishobora kwanduza ibyiza mubigeragezo.
Ubwoko butanu bwibicuruzwa bya PCR
Hariho impamvu nyinshi zo kwanduza PCR, zishobora kugabanywa mubice bikurikira:
Umwanda wanduye uterwa ahanini no kwanduza kontineri yo gukusanya icyitegererezo, cyangwa mugihe icyitegererezo gishyizwe, gisohoka muri kontineri kubera gufunga kashe, cyangwa icyitegererezo gifatirwa hanze yikintu, gitera kwanduzanya;Kwanduza biganisha ku kwanduza ingero;mikorobe zimwe na zimwe, cyane cyane virusi, zishobora gukwirakwizwa na aerosole cyangwa gukora aerosole, bikaviramo kwanduzanya.
Impamvu nyamukuru nuko mugihe cyo gutegura reagent ya PCR, imbunda ntangarugero, kontineri, amazi yatoboye kabiri nibindi bisubizo byandujwe nicyitegererezo cya PCR nucleic aside.
Muri laboratoire ya biologiya ya laboratoire na laboratoire zimwe na zimwe zikoresha plasmide ya clonide nkigenzura ryiza, ikibazo cyo kwanduza plasmide nacyo kirasanzwe.Kuberako ibikubiye muri clon plasmid mubunini bwibice biri hejuru cyane, kandi nibindi bikoresho na reagent birakenewe mugikorwa cyo kweza, kandi plasmide mungingo nzima irashobora kwanduzwa cyane kubera gukura gukomeye nubushobozi bwimyororokere ya selile nzima.
Kwanduza ibicuruzwa byongerewe imbaraga nikibazo gikunze kwanduzwa muri reaction ya PCR.Kuberako kopi yibicuruzwa bya PCR ari nini (muri rusange 1013 kopi / ml), ikaba irenze kure imipaka ya kopi ya kopi ya PCR, umubare muto cyane wibicuruzwa bya PCR bishobora gutera ibyiza.
Guhumanya ikirere ni uburyo bushoboka bwo kwanduza ibicuruzwa bya PCR, kandi nabwo byoroshye kwirengagizwa.Ikorwa no guterana hagati yubuso bwikirere nikirere.Mubisanzwe, kwanduza aerosol birashobora gukorwa mugihe umupfundikizo ufunguye, mugihe icyitegererezo cyifuzwa, cyangwa nigihe umuyoboro wa reaction uhungabana cyane.Ukurikije imibare, agace ka aerosol gashobora kuba kopi 48.000, bityo umwanda uterwa nacyo nikibazo gikwiye kwitabwaho bidasanzwe.
By'umwihariko, laboratoire yo gupima akenshi ikoresha primers imwe kugirango igerageze gene runaka.Igihe kirenze, umubare munini wibicuruzwa bya PCR bizagaragara muri laboratoire.Iyo umwanda umaze kubaho, biragoye kuwukuraho mugihe gito.
Kubwoko butatu bwambere bwumwanda, turashobora gukoresha uburyo bwiza bwo kwirinda, ariko umwanda uterwa nibicuruzwa bya PCR biragoye kubikumira, cyane cyane mukubaka laboratoire zidasanzwe za PCR.Mubikorwa bya PCR, mugihe umuyoboro wa pipette unywa kandi ugahuha amazi, hanyuma igifuniko cya PCR kigafungurwa, hazakorwa aerosol.Molekile ya ADN itwarwa na aerosol (aerosol imwe irashobora gutwara ADN ibihumbi icumi) biragoye kuyikuraho kuko ireremba mu kirere.Mugihe gikurikiraho cya PCR igeragezwa, ibyiza byibinyoma byanze bikunze bizabaho.
Nkuko bigaragara ku gishushanyo gikurikira, igenzura ribi naryo ryongereye umurongo uhuza inyungu:
Igice cya mbere cyiki kibazo cy’umwanda wa PCR no gukumira cyatangijwe hano.Ikibazo gikurikira kizakuzanira igice cya kabiri "Kwirinda ibicuruzwa byanduye PCR", komeza ukurikirane!
Igihe cyoherejwe: Nyakanga-25-2017
