Isesengura rikurikira ryibibazo bishoboka muriBuccalswab /FTA card DNA extraction is helpful to your experiment. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali:Tech@foregene.com.

Inkingi yo kweza ifunze

Muri iki gikoresho, mugikorwa cyo gukuramo ADN genomic, inkingi yo kweza iramenyeshwa neza kuri sample enzymatique lysis ivanze nta ntambwe ya centrifugation, kandi inkingi yo kweza irashobora guhagarikwa kubera imisemburo ituzuye hamwe nubukonje bwinshi bwikitegererezo.

Impamvu zikurikira zishoboka nizi zikurikira:

1. Igogorwa ryuzuye ryimisemburo yintangarugero.

Icyifuzo: Igihe cyo gutunganya icyitegererezo cya Foregene Protease gishobora kongerwa muburyo bukwiye cyangwa ndengakamere gishobora gufatwa nyuma ya centrifugation saa 12,000 rpm (~ 13,400× g) kuminota 5.

2. Gukoresha cyane ingero za tissue cyangwa tissue nini.

Icyifuzo: Nibyiza kutarenza 1Buccal swab in sample;niba icyitegererezo ari kinini cyane, ongera dosiye ya Buffer ST1, Protease ya Foregene, buffer ST2 ukurikije.

3. Icyitegererezo cyubwiza buri hejuru cyane.

Icyifuzo: Ingero zirashobora kuvangwa neza na mM 10 za Tris-HCl mbere yo gukuramo ADN genomic.

4. Ibice by'ikarita y'amaraso byanyoye.

Icyifuzo: Igihe cya centrifugation yinzibacyuho yintambwe ya 6 yumwanya wamaraso (Ikarita ya FTA) gukuramo genomic birashobora kwongerwa muburyo bukwiye.

Umusaruro muke cyangwa nta ADN

Hariho ibintu byinshi bigira ingaruka kumusaruro wa ADN genomic, harimo inkomoko yicyitegererezo, uburyo bwo kubika icyitegererezo, gutegura icyitegererezo, manipulation, nibindi.

ADN ya ADN ntishobora kuboneka mugihe cyo kuyikuramo

Impamvu zishoboka nizi zikurikira:

1. Kubika neza ingero cyangwa kubika igihe kirekire biganisha ku kwangirika kwa ADN.

Icyifuzo: Ibibyimba byo mu kanwa bigomba guhitamo icyitegererezo gishya, kandi ntabwo ari byiza gukoresha ibishishwa byabitswe mu bikorwa byo gukuramo ADN;icyitegererezo cyamaraso kigomba kwemeza ko ubuziranenge bujuje ibisabwa kandi igihe cyo kubika ntigikwiye kuba kirekire.

2. Gukoresha uduce duto cyane birashobora gutuma nta gukuramo ADN ikwiranye.

Icyifuzo: Kurikizabuccal swab sampling amabwiriza yubuyobozi bukora, hanyuma uhanagure inshuro nyinshi zishoboka kugirango selile zihagije zishobora kwomekwa kumunwa wo mu kanwa kugirango hakurwe ADN genomic;kubikuramo amaraso ntangarugero, ahantu haca amaraso harashobora kwiyongera muburyo bukwiye.

3. Foregene Protease ibitswe nabi, bigatuma igabanuka ryibikorwa byayo cyangwa kudakora.

Icyifuzo: Emeza uburyo bwo kubika bwathe F.oregene Protease cyangwa uyisimbuze na Protease nshya ya Foregene ya reaction ya enzymatique.

4. Kubungabunga bidakwiye ibikoresho cyangwa igihe cyo kubika ni birebire cyane, bikaviramo kunanirwa ibice bimwe mubikoresho.

Icyifuzo: Gura agashyaBuccal ADNkwigunga kit kubikorwa bijyanye.

5. Buffer WB ntabwo yongeyeho etanol yuzuye.

Icyifuzo: Emeza ko buffer WB yongeraho ingano yukuri ya Ethanol yuzuye.

6. Eluent ntabwo yongewe neza muri firime ya silicone.

Icyifuzo: Ongeraho 65° C.mbere yo gushyushya eluent itonyanga hagati ya silicone ya membrane hanyuma igasiga ubushyuhe bwicyumba kuminota 5 kugirango byongere imikorere ya elution.

Low-umusaruro ADN ADN mu bwigunge

Impamvu zikurikira zishoboka nizi zikurikira:

1. Kubika neza ingero cyangwa kubika igihe kirekire biganisha ku kwangirika kwa ADN.

Icyifuzo: Guswera mu kanwa nibyiza ko byatoranijwe bishya, kandi ibishishwa byabitswe ntibigomba gukoreshwa mugukuramo ADN genomic.

2. Niba ingano yintangarugero ya tissue ari nto cyane, ibice bya ADN byakuweho bizaba bike.

Icyifuzo: Kurikiza amabwiriza yo gutoranya umunwa wo mu kanwa mu gitabo gikora, uhanagura inshuro nyinshi zishoboka kugirango selile zihagije zishobora kwomekwa kumanwa yo gukuramo ADN genomic.

3. Foregene Protease ibitswe nabi, bigatuma igabanuka ryibikorwa byayo cyangwa kudakora.

Icyifuzo: Emeza uburyo bwo kubika bwathe Foregene Protease cyangwa kuyisimbuza ikindi gishya Foregene Protease ya reaction enzymatique.

4. Ibibazo bikomeye.

Icyifuzo: Koresha Buffer EB kugirango ukureho;niba ukoresha ddH₂O cyangwa izindi ntore, wemeze ko pH ya eluate iri hagati ya 7.0-8.5.

5. Intore ntabwo yongeweho neza neza.

Icyifuzo: Ongeramo ibitonyanga byoroshye hagati ya silicone ya membrane hanyuma usige ubushyuhe bwicyumba kuminota 5 kugirango wongere imikorere ya elution.

6. Amazi ya elution yegeranya bike cyane.

Icyifuzo: Koresha ubuhanga bwo gukuraho ADN genomic nkuko bisabwa mumabwiriza, byibuzentabwo ari munsi kurenza 15μl.

Low of ADNmu bwigunge

Ubushobozi buke bwa ADN bwa ADN bushobora gutera kunanirwa cyangwa ibisubizo bidashimishije byubushakashatsi bwo hasi, nka: enzymes ntishobora gucibwa, PCR ntishobora kubona agace ka gene yinyungu, nibindi.

Impamvu zishoboka nizi zikurikira:

1. Umwanda wa Heteroprotein, umwanda wa RNA.

Isesengura: Inkingi yo kweza ntabwo yogejwe hakoreshejwe Buffer PW;inkingi ya Buffer PW yoza isuku ntabwo yogejwe ikoresheje umuvuduko ukwiye wa centrifugal.

Icyifuzo: Menya neza ko nta mvura igwa muri supernatant mbere yo kongeramo Ethanol;menya neza koza inkingi yo kweza ukurikije amabwiriza, kandi iyi ntambwe ntishobora kuvaho.

2. Guhumanya ion.

Isesengura: Buffer WB yoza isuku inkingi yasibwe cyangwa yogejwe rimwe gusa, bivamo kwanduza ionic.

Icyifuzo: Witondere gukaraba Buffer WB inshuro 2 nkuko byateganijwe kugirango ukureho ion zisigaye bishoboka.

3. RNA yanduye.

Isesengura: RNase yamahanga yongewe kuri buffer;Buffer PW yo gukaraba ntiyari yo, bituma ibisigazwa bya RNase, bigira ingaruka kumikorere ya RNA yo hepfo, nko muri vitro transcription.

Icyifuzo: Foregene series nucleic asideisolaibikoresho bya tion birashobora gukuraho RNA nta yongeyeho RNase,bityo buccal Swab / Ikarita ya FTA Ikarita yo kwigungainshinge't Ongeraho RNase;witondere gukurikiza amabwiriza ya Buffer PW yoza inkingi, kandi iyi ntambwe ntishobora kuvaho.

4. Ibisigisigi bya Ethanol.

Isesengura: Buffer WB ntabwo yakoze ubusa ya centrifugation yubusa nyuma yo koza inkingi yo kweza.

Icyifuzo: Kora imikorere yubusa ya tube centrifugation ukurikije amabwiriza.

5. Ibindi byanduye.

Isesengura: Ingero zabitswe cyangwa ingero zidasanzwe ntiziteganijwe.

Icyifuzo: Witondere neza icyitegererezo nkuko byateganijwe.