Gukuramo aside nucleique nubuhanga bwo gutangiza ibinyabuzima bya molekuline.Niba ishobora kumenya no gukoresha ubu buhanga neza nayo yabaye icyangombwa kubashakashatsi nyuma kugirango bazamure abahanga mubushakashatsi.
Ukurikije ibikoresho bitandukanye byubushakashatsi, abashakashatsi bigabanyijemo amoko atatu akurikira:
Ibikoko byingenzi, Ibimera binini, Microbiology major
Buri bwoko bwabashakashatsi bazakomeza kwigana kopi kugirango bahingure ubuhanga bwihariye kuburugero rwihariye.
Uyu munsi, umwanditsi arashaka kuzana ibikoresho hamwe na porogaramu zitaboneka binyuze muri gereza kubashakashatsi.Hamwe nabo, urashobora kuzamura byihuse mubyerekezo byose.Ntabwo ari inzozi guhinduka imana igerageza.
Iki kibazo kireba abashakashatsi babigize umwuga (abagerageza indi myuga nabo barashobora kubyigiraho ~).
Nizera ko buri gisekuru cyabashakashatsi bazaba bumvise uburyo bwo kuvoma aside nucleic ya classique ya kera yagiye ikurikirana uko ibisekuruza byagiye bisimburana kubababanjirije.Amazina atatu manini munganda zikuramo ibihingwa.
Nubwo abasore bakomeye ari beza, baracyafite amakosa adashobora kwirindwa.
Uburyo bwa TRIzol
Akenshi bikoreshwa mugukuramo
RNA
Uburozi kandi butoroshye
● Harimo kwanduza genome
Uburyo bwa CTAB
Akenshi bikoreshwa mugukuramo
genomeADN
● Gutwara igihe
Biragoye gukuraho CTAB ukaraba kuruta umunyu, umubare muto wibisigara bizagira ingaruka zikomeye kubikorwa bya enzyme
Uburyo bwa SDS
Akenshi bikoreshwa mugukuramo
plasmid
● Ntibikwiye kubimera bifite polysaccharide nyinshi
● Kwanduza poroteyine
Muri iyi societe igezweho hamwe no kuvugurura byihuta inshuro ebyiri, uburyo gakondo bwo gukora ntibuba bugishoboye guhaza ibyifuzo byabashakashatsi, kandi kuzamura ibikoresho biregereje.
Foregene Nucleic Acide Extraction Kit yageragejwe N inshuro nabashakashatsi benshi.Nibyoroshye gukora, ntabwo ikoresha reagent yuburozi, kandi ntabwo yongeraho enzymes.Acide nucleic yabonetse ifite isuku nyinshi, ningirakamaro mubikorwa byo gukuramo burimunsi byabashakashatsi beza.
Foregene RNA Kwigunga ibikoresho
Hano, nzahishura kandi uburyo bwihishe bwo gukora kubashakashatsi, aribwo guhitamo uburyo bukwiye bwo kuvoma ukurikije ubwoko bwibihingwa (uburyo bwo kumenya ubwoko bwibihingwa biri kumpera yingingo!), Kuko bimwe mubihingwa polysaccharide na polifenole biri mubirimo, kandi uburyo gakondo burica.Ntabwo bihagije kugenzura.
Nibyiza, ni nkibi ...
Uburyo bwa TRIzol bwapfukamye munsi yintangarugero zifite polysaccharide nyinshi na polifenol nkibibabi byibitoki, amababi ya ginkgo namababi yipamba.
Kubwiyi ntego, Foregene yakoze ibikoresho bibiri bifatika kubashakashatsi.Igikoresho cyo gukuramo RNA gifite polysaccharide nkeya hamwe na polifenol hamwe na polysaccharide nyinshi hamwe na polifenol nyinshi bishobora kwica abanzi muburyo bugamije, kuburyo abashakashatsi batagitinya ibihingwa bitandukanye.Hindura gusa ibikoresho!
Usibye gukenera kuzamura uburyo bwo kuvoma, ibikorwa byintangiriro yo gutunganya nabyo bigomba kuvugururwa.
Kubera imiterere yihariye yicyitegererezo cyibimera, imizi nigiti gikomeye cyibiti bimwe na bimwe biragoye gusya no kumenagura neza.
Mu nganda zo gusya, abantu bagera kuri 80% bakoresha azote yuzuye yo gusya, mugihe abatageze kuri 20% bakoresha urusyo rwamashanyarazi no gusya amasaro.
Nubwo gusya kwa azote yuzuye bihendutse kandi byoroshye kuyikoresha, ninzozi mbi kubashakashatsi bafite icyitegererezo kinini cyo gusya buri gihe n'imbaraga!Niba minisiteri idasukuwe, bizatera kwanduzanya hagati yintangarugero.
Ingaruka zo gusya amashanyarazi ziratandukanye kubantu, kandi akamenyero ko gukora kubashakashatsi kazagira ingaruka zikomeye kubusugire bwa acide nucleique, kandi nibisabwa gukora ubushyuhe buke nibiba ngombwa.
rero, gusya amasaro yinyeganyeza birashobora gufatwa nkibikoresho byoroheje kandi byiza byo gusya.
Uburyo busanzwe bwo kunyeganyega burimo guhindagurika guhagaritse, gutambuka gutambitse gutambitse hamwe na bitatu-bine (gutambuka gutambitse).Nyamara, uruganda rwimitsi rwa Foregene ruciye kumurongo uriho hanyuma rugakoresha uburyo bushya bwa 4D bwo kunyeganyega kugirango urusyo rusa neza.
Kuzuza ibisabwa bitandukanye byicyitegererezo, byoroshye gukora
Ikoranabuhanga ridasanzwe rya 4D, guhonyora byiyongereyeho 20%
Icyitegererezo gifunze rwose, nta kwanduzanya
Hatuje, ≤ 56 decibels
Gereranya ibisubizo byubushakashatsi n'umuraba ~
Icyitegererezo: 50mg amababi yingano (ibirimo bike bya polysaccharide na polifenol)
Gukuramo ubwoko bwa acide nucleic: RNA
Umubare wikibanza: 5μl
Ibikoresho byo gukuramo byakoreshejwe ni Foregene Igihingwa Cyuzuye RNA Ikuramo (RE-05011)
Ugereranije amakuru hamwe nigishushanyo cya electrophoreis, urashobora kubona ko kwibumbira hamwe nubuziranenge bwa RNA yakuwe nyuma yo gusya imashini birenze ibyo gusya kwa azote.Muri icyo gihe, kubera ko gusya imashini bishobora kumena icyitegererezo burundu, RNA nto irashobora gukururwa neza kandi ubusugire bwa RNA burashobora gukomeza.
Icyitegererezo: 50mg amababi ya ginkgo (polysaccharide nyinshi hamwe na polifenol)
Gukuramo ubwoko bwa acide nucleic: RNA
Umubare wikibanza: 5μl
Gukoresha Foregene Polysaccharide Igiti cya Polifenol Igikoresho cyose cyo gukuramo RNA (RE-05021)
Reba kuri iri tsinda ryintangarugero zirimo ibintu byinshi bya polysaccharide na polifenol.Ingaruka iragaragara cyane.Hamwe nubuziranenge bumwe, ubunini bwo gusya imashini RNA ikubye hafi inshuro 5 gusya kwa azote yuzuye, kandi RNA nto iracyagaragara neza ~
Niba wumva ko ntanimwe mubikoresho byavuzwe haruguru byihuta bihagije, noneho umwanditsi agomba kuzana ibikoresho byanyuma!
Tera urukurikirane rwa PCR
Umwihariko wibi bikoresho nuko amababi yimbuto cyangwa imbuto bidakenera kuba hasi no kumeneka, kandi bikajugunywa mu muyoboro wa EP kugirango lysis, kandi ivangwa rya lysis bivamo rishobora gukoreshwa nkicyitegererezo cya PCR.Nibyoroshye nkintambwe imwe byihuse!Muri icyo gihe, uruhererekane rwa PCR rw’ibimera bya Foregene rufite gahunda ya UNG yo kurwanya umwanda, ku buryo abashakashatsi batagifite impungenge z’umwanda wa aerosol muri laboratoire kandi ushobora gukora ubushakashatsi byoroshye.
Inama zurubanza:
Nigute ushobora kumenya niba ari igihingwa cya polysaccharide polifenol
Fata hafi ya 20mg ya tissue hanyuma uyishyire mumiyoboro isukuye ya 1.5ml centrifuge, ongeramo 200ul 200mM NaOH igisubizo, ubishyire kuri 95 ℃ kuminota 10min, urebe ihinduka ryamabara nyuma ya lysis
1. Niba ibara rya lysate ari icyatsi cyangwa umuhondo wijimye, bivuze ko ibirimo tissue polifenol ari bike, kandi birasabwa gukoresha urukurikirane rwa Foregene Plant
2. Niba lysate ari umukara-umuhondo cyangwa umutuku-umutuku, bivuze ko ibice bya polifenol biri hejuru, kandi birasabwa gukoresha Foregene Polysaccharide Polyphenol Plant Plus Series
Igihe cyo kohereza: Kanama-20-2021
