Uracyakoresha Trizol kugirango ukure RNA?
Gosh, ntundeke mpangayikishijwe n'ubuzima bwawe!
Trizol iri hafi guhezwa, ntumbwire ko ukiri mu mwijima?

Ikintu nyamukuru kigize Trizol reagent ni fenol, kandi reagent kama nka chloroform nayo yongewe mubikorwa byubushakashatsi.Kumara igihe kirekire bizangiza ubuzima bwawe, cyane cyane guhura kwuruhu birashobora gutera imiti ikaze ndetse ninkovu zihoraho.

Mubihe bisanzwe, abafatanyabikorwa ba laboratoire barashobora kugabanya gusa ibyago bitwaje intwaro zose (bambaye amakoti ya laboratoire, gants na masike).

Kandi inama nakugira nuko: nyamuneka usimbuze Trizol kugirango wirinde ingaruka zituruka.

1. Igisekuru cyambere cyibicuruzwa bya RNA
Sohoka muri 1987 (Trizol reagents)

akarusho:
Gukuramo byinshi

Ibibi:
Uburozi bwangiza nka guanidine isothiocyanate-phenol-chloroform irakenewe;
Igikorwa kitoroshye;
Ukeneye gukoresha DNase kugirango ukureho ADN yanduye

2.Igisekuru cya kabiri ibicuruzwa byo gukuramo RNA
Sohoka muri 1994 (QIAGEN RNeasy nibicuruzwa byinshi murugo)

akarusho:
Inkingi centrifugal uburyo bwakoreshejwe, bworoshye gukora;

Ibibi:
Ukeneye centrifuge kugirango ukureho imyanda
Ukeneye gukoresha DNase kugirango ukureho ADN yanduye

3.Igisekuru cya gatatu ibicuruzwa byo gukuramo RNA
Gusohoka muri 2005 (QIAGEN RNeasy Yongeyeho na F.oregeneIbicuruzwa bikurikirana bya RNA)

akarusho:
Koresha mu buryo butaziguye inkingi yo gukuraho ADN kugirango ukureho ADN hamwe n’imyanda icyarimwe, ukoreshe igihe n'imbaraga;
Umutekano kandi woroshye, nta mpamvu yo kongeramo akayunguruzo hamwe na DNase kugirango ukureho umwanda byoroshye kandi wirinde kwanduza ADN

Reka turebe itandukaniro ryimikorere hagati yibisekuru bya mbere (Trizol), igisekuru cya kabiri (RNeasy) nibicuruzwa bya gatatu (Foregene) byo gukuramo RNA:

Igikorwa mumashusho kirasobanutse: igisekuru cya gatatu ibicuruzwa bifite ibyiza bigaragara

Amagambo atatu gusa yo gusobanura: byoroshye!Byihuta!Umutekano

Imbonerahamwe ya PK y'ibisubizo byubushakashatsiKuriigisekuru cya mbere, ibisekuru bya kabiri nibisekuru bya gatatu byo gukuramo RNA:

1. EYamazaki

Igisekuru cya mbere (1: Trizol)
Igisekuru cya kabiri (2: QIAGEN RNeasy)
Igisekuru cya gatatu (3: QIAGEN RNeasy plus, 4: Foregene)

Ni ubuhe bwoko bw'ibicuruzwa ari byiza, uzabimenya ugereranije
RNA yakuweho ikoresheje ibikoresho bya Foregene
Ntabwo kwibanda cyane ahubwo no kuba inyangamugayo nziza

Igishushanyo cya 2.qPCR (igishushanyo mbonera cya amplification)

Igisekuru cya mbere (Trizol)

Igisekuru cya kabiri (ikirere cyubururubisobanuraQIAGEN RNeasy-, umutukubisobanuraQIAGEN RNeasy +)

Igisekuru cya gatatu (ubururu bisobanura Foregene, icyatsi bisobanura QIAGEN RNeasy plus)

.

Yaba electrophoretogramu cyangwa ingano ya PCR amplification curve igereranya, ubwiza bwibicuruzwa bya Foregene ntabwo biri munsi yibicuruzwa byatumijwe mu mahanga.