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Uwakoze Ubushinwa PCR Laboratoire Nucleic Acide Sisitemu (Sharon)

Ibisobanuro:

2X Real PCR YoroshyeTMKuvanga-SYBR yatanzwe nigihe nyacyo PCR ByoroshyeTM-SYBR Icyatsi I kit ni sisitemu nshya ya premix ikoresha SYBR Icyatsi I kuri Real Time PCR amplification reaction, ishobora kuzamura cyane umwihariko wibicuruzwa no kubyumva neza.ROX itangwa nk'irangi ryo kugenzura imbere.Ubwinshi bwa fluorescence yiki gikoresho bwikubye inshuro 3-5 ibyo bicuruzwa bisa, birashobora kuba byoroshye kandi bikagaragaza ubushishozi bwibanze bwa ADN yerekana icyitegererezo.

2X PCR YukuriTMKuvanga-SYBR irimo Foregene idasanzwe ishyushye-itangira Taq ADN Polymerase.Ugereranije na misemburo isanzwe ya Taq, ifite ibyiza byo gukora neza cyane, ubushobozi bwihariye bwo kongera imbaraga hamwe nigipimo gito kidahuye.Irashobora kugabanya amplification idasanzwe no kunoza ukuri kwa PCR.


Ibicuruzwa birambuye

Ibicuruzwa

Guhanga udushya, byiza kandi byizewe nindangagaciro shingiro yikigo cyacu.Aya mahame uyumunsi kuruta ikindi gihe cyose aribwo shingiro ryibyo twagezeho nkumuryango mpuzamahanga ukora hagati yubucuruzi buciriritse bukora uruganda rukora Laboratoire y'Ubushinwa PCRSisitemu yo gukuramo aside Nucleic.
Guhanga udushya, byiza kandi byizewe nindangagaciro shingiro yikigo cyacu.Aya mahame uyumunsi kuruta ikindi gihe cyose shingiro ryibyo twagezeho nkumuryango mpuzamahanga ukora hagati yubucuruzi buciriritse kuriUbushinwa Nucleic Acide, Sisitemu yo gukuramo aside Nucleic, Dufite injeniyeri zo hejuru muri izi nganda hamwe nitsinda ryiza mubushakashatsi.Ikirenzeho, ubu dufite archives zacu umunwa n'amasoko mubushinwa ku giciro gito.Kubwibyo, turashobora guhura nibibazo bitandukanye kubakiriya batandukanye.Ugomba gushaka urubuga rwacu kugirango ugenzure amakuru menshi kubicuruzwa byacu nibisubizo.

Ibisobanuro

5ml, 10ml, 40ml

Ibigize ibikoresho

0.1U Intwari ya Taq / μl

400μM DNTP

20mM Tris-HCl (pH8.8)

100mM KCl

5mM MgCl2

Izindi stabilisateur hamwe nabazamura
Ububiko bwa ADN

Ibiranga ibyiza

Ubudahemuka buhebuje: inshuro 6 z'umusemburo wa Taq usanzwe;

Speed ​​Umuvuduko wihuse wihuta: umuvuduko wa amplification ni 5-10sec / kb, wikubye inshuro 6-12 ugereranije na Taq enzyme isanzwe;

Ibindi byitegererezo byo guhuza n'imiterere: Irashobora kwagura neza inyandikorugero zitandukanye za ADN hamwe na hejuruIbirimo GC;

Efficiency Gukora neza cyane: kugabanuka kwinshi kurenza imisemburo ya Taq isanzwe;

Erance Kwihanganira ibidukikije birakomeye: bishyirwa kuri 37 ° C icyumweru, bikomeza ibikorwa birenga 90%;

Ifite ibikorwa 5 ′ → 3 ′ ibikorwa bya ADN polymerase na 5 ′ → 3 ′ ibikorwa bya exonuclease, nta 3 ′ → 5 ′ ibikorwa bya exonuclease.

Kit Ibikoresho byabanjirijwe kuvangwa na Electrophoresis Loading Buffer, PCR imaze kurangira, icyitegererezo gishobora gutwarwa na electrophorei;imikorere iroroshye kandi yoroshye.

Porogaramu

Kwiyongera kwa PCR ibice bya ADN, ibimenyetso bya ADN, uko ADN ikurikirana, PCR wongeyeho umurizo

Ububiko hamwe nubuzima bwa Shelf

Irashobora kubikwa igihe kirekire kuri -20 ° C;kubikoresha kenshi, birashobora kubikwa kuri 4 ° C mugihe gito.Guhanga udushya, byiza kandi byizewe nindangagaciro yibanze yikigo cyacu.Aya mahame uyumunsi kuruta ikindi gihe cyose aribwo shingiro ryibyo twagezeho nkumuryango mpuzamahanga ukora hagati yubucuruzi buciriritse bukora uruganda rukora Ubushinwa Biobase Bnp32 Laboratoire ya PCRSisitemu yo gukuramo aside Nucleic.
Uruganda rwaUbushinwa Nucleic AcideSisitemu yo gukuramo Nucleic Acide, Dufite injeniyeri zo hejuru muri izi nganda hamwe nitsinda ryiza mubushakashatsi.Ikirenzeho, ubu dufite archives zacu umunwa n'amasoko mubushinwa ku giciro gito.Kubwibyo, turashobora guhura nibibazo bitandukanye kubakiriya batandukanye.Ugomba gushaka urubuga rwacu kugirango ugenzure amakuru menshi kubicuruzwa byacu nibisubizo.


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