Uwakoze Ubushinwa PCR Laboratoire Nucleic Acide Sisitemu (Sharon)
Guhanga udushya, byiza kandi byizewe nindangagaciro shingiro yikigo cyacu.Aya mahame uyumunsi kuruta ikindi gihe cyose aribwo shingiro ryibyo twagezeho nkumuryango mpuzamahanga ukora hagati yubucuruzi buciriritse bukora uruganda rukora Laboratoire y'Ubushinwa PCRSisitemu yo gukuramo aside Nucleic.
Guhanga udushya, byiza kandi byizewe nindangagaciro shingiro yikigo cyacu.Aya mahame uyumunsi kuruta ikindi gihe cyose shingiro ryibyo twagezeho nkumuryango mpuzamahanga ukora hagati yubucuruzi buciriritse kuriUbushinwa Nucleic Acide, Sisitemu yo gukuramo aside Nucleic, Dufite injeniyeri zo hejuru muri izi nganda hamwe nitsinda ryiza mubushakashatsi.Ikirenzeho, ubu dufite archives zacu umunwa n'amasoko mubushinwa ku giciro gito.Kubwibyo, turashobora guhura nibibazo bitandukanye kubakiriya batandukanye.Ugomba gushaka urubuga rwacu kugirango ugenzure amakuru menshi kubicuruzwa byacu nibisubizo.
Ibisobanuro
5ml, 10ml, 40ml
Ibigize ibikoresho
0.1U Intwari ya Taq / μl |
400μM DNTP |
20mM Tris-HCl (pH8.8) |
100mM KCl |
5mM MgCl2 |
Izindi stabilisateur hamwe nabazamura |
Ububiko bwa ADN |
Ibiranga ibyiza
Ubudahemuka buhebuje: inshuro 6 z'umusemburo wa Taq usanzwe;
Speed Umuvuduko wihuse wihuta: umuvuduko wa amplification ni 5-10sec / kb, wikubye inshuro 6-12 ugereranije na Taq enzyme isanzwe;
Ibindi byitegererezo byo guhuza n'imiterere: Irashobora kwagura neza inyandikorugero zitandukanye za ADN hamwe na hejuruIbirimo GC;
Efficiency Gukora neza cyane: kugabanuka kwinshi kurenza imisemburo ya Taq isanzwe;
Erance Kwihanganira ibidukikije birakomeye: bishyirwa kuri 37 ° C icyumweru, bikomeza ibikorwa birenga 90%;
Ifite ibikorwa 5 ′ → 3 ′ ibikorwa bya ADN polymerase na 5 ′ → 3 ′ ibikorwa bya exonuclease, nta 3 ′ → 5 ′ ibikorwa bya exonuclease.
Kit Ibikoresho byabanjirijwe kuvangwa na Electrophoresis Loading Buffer, PCR imaze kurangira, icyitegererezo gishobora gutwarwa na electrophorei;imikorere iroroshye kandi yoroshye.
Porogaramu
Kwiyongera kwa PCR ibice bya ADN, ibimenyetso bya ADN, uko ADN ikurikirana, PCR wongeyeho umurizo
Ububiko hamwe nubuzima bwa Shelf
Irashobora kubikwa igihe kirekire kuri -20 ° C;kubikoresha kenshi, birashobora kubikwa kuri 4 ° C mugihe gito.Guhanga udushya, byiza kandi byizewe nindangagaciro yibanze yikigo cyacu.Aya mahame uyumunsi kuruta ikindi gihe cyose aribwo shingiro ryibyo twagezeho nkumuryango mpuzamahanga ukora hagati yubucuruzi buciriritse bukora uruganda rukora Ubushinwa Biobase Bnp32 Laboratoire ya PCRSisitemu yo gukuramo aside Nucleic.
Uruganda rwaUbushinwa Nucleic AcideSisitemu yo gukuramo Nucleic Acide, Dufite injeniyeri zo hejuru muri izi nganda hamwe nitsinda ryiza mubushakashatsi.Ikirenzeho, ubu dufite archives zacu umunwa n'amasoko mubushinwa ku giciro gito.Kubwibyo, turashobora guhura nibibazo bitandukanye kubakiriya batandukanye.Ugomba gushaka urubuga rwacu kugirango ugenzure amakuru menshi kubicuruzwa byacu nibisubizo.