Icyo twibandaho gikwiye kuba uguhuriza hamwe no kuzamura ubwiza no gusana ibicuruzwa biriho, hagati aho buri gihe gushiraho ibicuruzwa bishya kugirango duhuze abakiriya badasanzwe 'kubisabwa byiza Ubushinwa Inkingi Nucleic Acide Rna Isolation Kit, igisekuru cya gatatu, Buri kimwe mubitekerezo n'ingamba bigiye gushimwa cyane!Ubufatanye bwiza bushobora gutera imbere twembi mukuzamuka neza!
Ibyo twibandaho bigomba kuba ugushimangira no kuzamura ubwiza no gusana ibicuruzwa biriho, hagati aho guhora dushiraho ibicuruzwa bishya kugirango duhuze abakiriya badasanzwe bakeneye.Ubushinwa, Pcr Rna Gukuramo Reagent, Twubahiriza ubunyangamugayo, bukora neza, bufatika bwunguka-gutsindira ubutumwa hamwe na filozofiya yubucuruzi ishingiye kubantu.Ubwiza buhebuje, igiciro cyiza no guhaza abakiriya burigihe bikurikiranwa!Niba ushishikajwe nibintu byacu, gerageza utwandikire kubindi bisobanuro!

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Ibigize ibikoresho

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Icyo twibandaho ni ugushimangira no kuzamura ubuziranenge no gusana ibicuruzwa biriho ubu, hagati aho buri gihe hagashyirwaho ibicuruzwa bishya kugira ngo byuzuze abakiriya badasanzwe 'kubisabwa byiza Ubushinwa Magnetic Particles Nucleic Acide Rna Isolation Kit, Buri kimwe mubitekerezo n'ingamba bigiye gushimwa cyane!Ubufatanye bwiza bushobora gutera imbere twembi mukuzamuka neza!
Ubwiza bwizaUbushinwa, PCR Rna Gukuramo Reagent, Twubahiriza ubunyangamugayo, bukora neza, bufatika bwo gutsindira inyungu hamwe na filozofiya yubucuruzi.Ubwiza buhebuje, igiciro cyiza no guhaza abakiriya burigihe bikurikiranwa!Niba ushishikajwe nibintu byacu, gerageza utwandikire kubindi bisobanuro!