Ubushinwa bukora Ubushinwa M48 Nucleic Acide Gukuramo ibikoresho Gukora no gutanga |Foregene

Uwakoze Ubushinwa M48 Nucleic Acide Gukuramo ibikoresho

Uwakoze Ubushinwa M48 Gukuramo Acide Nucleic Acide Igikoresho cyerekanwe

Ibisobanuro:

Iki gikoresho gikoresha sisitemu yihariye ya lysis kugirango irekure vuba ADN ya genomic kuva mubitangangingo byinyamanswa kubitekerezo bya PCR, kubwibyo birakwiriye cyane cyane kwipimisha rinini.

Igikorwa cyo kurekura ADN genomic muri lysis buffer cyarangiye muminota 10-30 kuri 65°C. Ntayindi nzira nka protein na RNA ikurwaho bisabwa, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.

2× PCR Biroroshye^TMKuvanga bifite kwihanganira bikomeye PCR yerekana inhibitor, kandi irashobora gukoresha lysate yicyitegererezo kugirango igeragezwe nkicyitegererezo cyo gukora neza kandi cyihariye.Iyi reagent irimo Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, reaction ya reaction, optimizer ya PCR na stabilisateur.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ubucuruzi bwacu bushimangira ubuyobozi, kwinjiza abakozi bafite impano, no kubaka amatsinda, duharanira cyane kuzamura imyumvire n’inshingano z’abakozi.Isosiyete yacu yatsindiye neza IS9001 Icyemezo hamwe nu Burayi bwa CE Icyemezo cyo gukora ibicuruzwa mu Bushinwa M48 Nucleic Acide Extraction kit, Kubindi bisobanuro cyangwa niba ufite ikibazo kijyanye nibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira.
Ubucuruzi bwacu bushimangira ubuyobozi, kwinjiza abakozi bafite impano, no kubaka amatsinda, duharanira cyane kuzamura imyumvire n’inshingano z’abakozi.Isosiyete yacu yatsindiye neza IS9001 Icyemezo na Europe CE Icyemezo cyaAcide Nucleic Acide, Ubushinwa Nucleic Acide, Duhuza ibyiza byacu byose kugirango dukomeze guhanga udushya, kunoza no kunoza imiterere yinganda no gukora ibicuruzwa.Tugiye guhora twemera kandi tuyikore.Murakaza neza kwifatanya natwe kumenyekanisha urumuri rwatsi, hamwe tuzakora ejo hazaza heza!

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.	Buffer AL
	Foregene Protease
	6 × ADN Yipakurura Buffer
Igice cya II	2 × PCR Biroroshye^TMKuvanga

Ibiranga ibyiza

■ Ntabwo bitwara igihe kandi bihenze bya ADN

Require Icyitegererezo gisabwa ni gito, nkibice 5mg byinyamanswa bishobora kugeragezwa.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ibipimo by'ibikoresho

Gushyira mu bikorwa: inyama zinyamaswa zitandukanye.

ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.

Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.

Urujya n'uruza rw'akazi

wk flw-inyamanswa yinyamanswa PCR

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.

Buffer AL reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.

Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.

Reagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.

Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.

Reagent 2 × PCR Biroroshye^TMImvange irashobora kubikwa amezi 12 kuri -20 ℃;niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ℃ kubikwa igihe gito (koresha muminsi 10).

Ubucuruzi bwacu bushimangira ubuyobozi, kwinjiza abakozi bafite impano, no kubaka amatsinda, duharanira cyane kuzamura imyumvire n’inshingano z’abakozi.Isosiyete yacu yatsindiye neza IS9001 Icyemezo hamwe nu Burayi CE Icyemezo cyo gukora Ubushinwa M48 Sisitemu yo Gukuramo Acide Nucleic Acide, Kubindi bibazo cyangwa ufite ikibazo kijyanye nibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira.
Uruganda rwaAcide Nucleic Acide, Ubushinwa Nucleic Acide, Duhuza ibyiza byacu byose kugirango dukomeze guhanga udushya, kunoza no kunoza imiterere yinganda no gukora ibicuruzwa.Tugiye guhora twemera kandi tuyikore.Murakaza neza kwifatanya natwe kumenyekanisha urumuri rwatsi, hamwe tuzakora ejo hazaza heza!

Mbere: Urupapuro rwibiciro kubushinwa Professional Magnetic Virus ADN / Rna Gukuramo Kit Nucleic Acide Isukura Reagent

Ibikurikira: Utanga Ubushinwa Fluorescent Nshya Rt-PCR Uburyo bwa Nucleic Acide Kumenya Ibikoresho byo Kwipimisha

Andika ubutumwa bwawe hano hanyuma utwohereze