Intego yacu ni uguhaza abakiriya bacu mugutanga inkunga ya zahabu, agaciro karenze hamwe nubwiza buhanitse kubakora inganda zambere mubushinwa Viral Rna & DNA Extraction Kit ya Spin Column Method, Turizera rwose ko tuzaguha hamwe nisosiyete yawe intangiriro nziza.Niba hari icyo tuzakora kugirango duhuze ibyo ukeneye, tuzaba twongeyeho kuruta kwishimira kubikora.Murakaza neza kubikorwa byacu byo gukora kugirango duhagarare by.
Intego yacu ni uguhaza abakiriya bacu mugutanga inkunga ya zahabu, agaciro keza kandi keza kuriUbushinwa Rna & ibikoresho byo gukuramo ADN, Gukuramo Acide Nucleic, Intego yacu ni ugufasha abakiriya kumenya intego zabo.Twakomeje gukora ibishoboka byose kugirango tugere kuri iki kibazo cyo gutsindira inyungu kandi turabashimira byimazeyo ko twifatanya natwe.Mu ijambo, iyo uduhisemo, uhitamo ubuzima butunganye.Murakaza neza gusura uruganda rwacu kandi mwakire neza ibyo mwategetse!Kubindi bisobanuro, ntugomba gutindiganya kutwandikira.

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo mer- mercaptoethanol (bidashoboka) .Intego yacu ni uguhora duhaza abakiriya bacu mugutanga inkunga ya zahabu, agaciro keza hamwe nubwiza buhanitse kubayobozi bambere bayobora Ubushinwa Viral Rna & DNA Extraction Kit ya Spin Column Method, Turizera rwose ko tuzaguha hamwe nisosiyete yawe intangiriro nziza.Niba hari icyo tuzakora kugirango duhuze ibyo ukeneye, tuzaba twongeyeho kuruta kwishimira kubikora.Murakaza neza kubikorwa byacu byo gukora kugirango duhagarare by.
Inganda zikora mubushinwa Rna & ADN ikuramo ibikoresho,Gukuramo Acide Nucleic, Intego yacu ni ugufasha abakiriya kumenya intego zabo.Twakomeje gukora ibishoboka byose kugirango tugere kuri iki kibazo cyo gutsindira inyungu kandi turabashimira byimazeyo ko twifatanya natwe.Mu ijambo, iyo uduhisemo, uhitamo ubuzima butunganye.Murakaza neza gusura uruganda rwacu kandi mwakire neza ibyo mwategetse!Kubindi bisobanuro, ntugomba gutindiganya kutwandikira.