Wibuke "Umukiriya ubanza, Byiza Byambere" mubitekerezo, dukorana cyane nabakiriya bacu kandi tukabaha serivisi nziza kandi zinzobere kubicuruzwa byinshi byuruganda.Ubushinwa Gukuramo Acide Nucleic ReagentIkizamini cyo gukuramo Rna, Ikigo cyacu cyiyemeje guha abakiriya ibintu byiza kandi byizewe byo mu rwego rwo hejuru ku giciro cyo gupiganwa, byinjiza buri mukiriya anyuzwe na serivisi zacu.
Wibuke "Umukiriya ubanza, Byiza cyane" mubitekerezo, dukorana cyane nabakiriya bacu kandi tubaha serivisi nziza kandi zinzobere kuriUbushinwa Gukuramo Acide Nucleic Reagent, Ibintu byacu bizwi cyane kandi byizewe nabakoresha kandi birashobora gukomeza guhora biteza imbere mubukungu nubukungu.Twakiriye neza abakiriya bashya kandi bashaje baturutse imihanda yose kugirango batubwire umubano wubucuruzi ndetse no kugera kubitsinzi!

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi nziza-yuzuye ya RNA ivuye mu ngirabuzimafatizo zifite 96, 24, 12, na 6-nziza.

Igikoresho gitanga ADN isukura neza, ishobora gutandukanya byoroshye na lysate ndengakamere, guhuza no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe.

Inkingi ya RNA yonyine irashobora guhuza neza RNA hamwe na formula idasanzwe.Umubare munini wintangarugero urashobora gutunganyirizwa icyarimwe.

Ibigize ibikoresho

* Nyamuneka wambare uturindantoki kandi ufate ingamba zo kubarinda mugihe cyo kubaga kuko Buffer cRL1 na Buffer RW1 zirimo umunyu wa chaotropique.

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

Igishushanyo

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-ethanethiol (itabishaka) .Byita "Umukiriya ubanza, Uwambere wambere" mubitekerezo, dukorana cyane nabakiriya bacu kandi tukabaha serivise nziza kandi zinzobere kubicuruzwa byinshi byo mu ruganda Ubushinwa Nucleic Acide Extraction Reagent (Serum / Plasma na Puncture solution) Kuzuza ibicuruzwa byiza hamwe na hamwe, buri mukiriya anyuzwe na serivisi zacu.
Uruganda rwogucuruza Ubushinwa Nucleic Acide Gukuramo Reagent, Uburyo bwa Magnetic Bead Method, Ibintu byacu biramenyekana cyane kandi byizewe nabakoresha kandi birashobora gukomeza guteza imbere ibikenewe mubukungu n'imibereho myiza.Twakiriye neza abakiriya bashya kandi bashaje baturutse imihanda yose kugirango batubwire umubano wubucuruzi ndetse no kugera kubitsinzi!