Ubu dufite abakozi benshi bakomeye bakoresha abakozi beza mubucuruzi bwa interineti, QC, no gukorana nubwoko bwikibazo giteye ikibazo muburyo bwo gukora uruganda rwubusaUbushinwa Rnasin Inhibitor, Uruganda rwacu rukora hamwe nihame ryuburyo bwa "bushingiye ku bunyangamugayo, ubufatanye bwashyizweho, abantu berekeza, ubufatanye-bunguka".Turizera ko byoroshye kugirana ubufatanye bushimishije numucuruzi uturutse ibidukikije.
Ubu dufite abakozi benshi bakomeye bakoresha abakozi beza mubucuruzi bwa interineti, QC, no gukorana nubwoko bwibibazo bitoroshye muburyo bwo gukoraUbushinwa Rnasin Inhibitor, Rnasin, Ibibazo byinshi hagati yabatanga nabakiriya biterwa no gutumanaho nabi.Mu mico, abatanga ibicuruzwa barashobora kwanga kubaza ibintu batumva.Turasenya izo nzitizi kugirango tumenye ibyo ushaka kurwego utegereje, mugihe ubishaka.Igihe cyo gutanga vuba nibicuruzwa ushaka ni Ibipimo byacu.

Ibisobanuro

5ml, 10ml, 40ml

PCR Intwari (Hamwe Irangi) Ibikoresho byo gukuramo birimo Foregene idasanzwe idasanzwe ya Taq - Foregene Taq Intwari, optime ya DNTPs, MgCl2, buffer reaction, PCR itera imbaraga, optimizer na stabilisateur.Sisitemu ya 2 × PCR HeroTM ivanze (irangi) ifite kwihanganira cyane inhibitori za PCR kuruta sisitemu isanzwe ya PCR ivanze, kandi irashobora guhangana byoroshye na PCR yogukwirakwiza inyandikorugero zitandukanye.Sisitemu idasanzwe ya reaction hamwe nubushobozi buhanitse Taq Intwari ituma reaction ya PCR igira ubushobozi bwo kongera amplification, umwihariko hamwe na sensitivite.

Ibigize ibikoresho

Ibiranga ibyiza

Ubudahemuka buhebuje: inshuro 6 z'umusemburo wa Taq usanzwe;

Speed ​​Umuvuduko wihuse wihuta: umuvuduko wa amplification ni 5-10sec / kb, wikubye inshuro 6-12 ugereranije na Taq enzyme isanzwe;

Ibindi byitegererezo byo guhuza n'imiterere: Irashobora kwagura neza inyandikorugero zitandukanye za ADN hamwe na hejuruIbirimo GC;

Efficiency Gukora neza cyane: kugabanuka kwinshi kurenza imisemburo ya Taq isanzwe;

Erance Kwihanganira ibidukikije birakomeye: bishyirwa kuri 37 ° C icyumweru, bikomeza ibikorwa birenga 90%;

Ifite ibikorwa 5 ′ → 3 ′ ibikorwa bya ADN polymerase na 5 ′ → 3 ′ ibikorwa bya exonuclease, nta 3 ′ → 5 ′ ibikorwa bya exonuclease.

Kit Ibikoresho byabanjirijwe kuvangwa na Electrophoresis Loading Buffer, PCR imaze kurangira, icyitegererezo gishobora gutwarwa na electrophorei;imikorere iroroshye kandi yoroshye.

Porogaramu

Kwiyongera kwa PCR ibice bya ADN, ibimenyetso bya ADN, uko ADN ikurikirana, PCR wongeyeho umurizo

Ububiko hamwe nubuzima bwa Shelf

Irashobora kubikwa igihe kirekire kuri -20 ° C;kugirango ukoreshwe kenshi, irashobora kubikwa kuri 4 ° C mugihe gito.Ubu dufite abakozi benshi bakomeye bakoresha neza cyane mugucuruza interineti, QC, no gukorana nubwoko bwibibazo bitera ibibazo muburyo bwo gukora uruganda rwubusa UrugandaRnasinInhibitor, Ikigo cyacu gikora hamwe nihame ryuburyo bwa "bushingiye ku butabera, ubufatanye bwashyizweho, abantu berekeza, ubufatanye-bunguka".Turizera ko byoroshye kugirana ubufatanye bushimishije numucuruzi uturutse ibidukikije.
Uruganda ntangarugero UbushinwaRnasinInhibitor, Rnasin, Ibibazo byinshi hagati yabatanga nabakiriya biterwa no gutumanaho nabi.Mu mico, abatanga ibicuruzwa barashobora kwanga kubaza ibintu batumva.Turasenya izo nzitizi kugirango tumenye ibyo ushaka kurwego utegereje, mugihe ubishaka.Igihe cyo gutanga vuba nibicuruzwa ushaka ni Ibipimo byacu.