Kugira ngo duhuze ibyifuzo byabakiriya byateganijwe, ubu dufite abakozi bacu bakomeye kugirango batange ubufasha bukomeye burimo ibicuruzwa, kugurisha, gutegura, gukora, kugenzura ubuziranenge bwo hejuru, gupakira, ububiko hamwe nibikoresho byo muruganda byakozwe bishyushye-kugurisha Magnetic Bead Nucleic Acide Gukuramo ibikoresho Rna Kwigunga ADN Isukura PCR Ikizamini, "Hindura kuri byinshi byiza!"ni intero yacu, bisobanura ngo “Isi nziza iri imbere yacu, reka rero tuyikunde!”Hindura kuri byinshi byiza!Mwese muriteguye?
Kugirango duhuze ibyifuzo byabakiriya byateganijwe, ubu dufite abakozi bacu bakomeye kugirango batange ubufasha bukomeye burimo ibicuruzwa, kugurisha, igenamigambi, umusaruro, kugenzura ubuziranenge bwo hejuru, gupakira, ububiko hamwe nibikoresho byaUbushinwa Magnetic Isaro hamwe nigitabo cyo gusuzuma, Ubunararibonye bwacu butugira ingenzi mumaso yabakiriya bacu.Ubwiza bwacu buvuga ubwabwo imitungo nkiyi idahindagurika, isuka cyangwa isenyuka, ibyo rero nibyo abakiriya bacu bazahora bizeye mugihe batanga itegeko.

Ibisobanuro

Iki gikoresho gikoresha ADN yonyine inkingi ishobora guhuza ADN, protease ya Foregene na sisitemu idasanzwe.ADN yo mu rwego rwohejuru irashobora gukurwa mu ngirabuzimafatizo zitandukanye zifite imico n’inyamanswa mu minota 30 kugeza kuri 50.

ADN yonyine ya silika gel membrane ikoreshwa muri spin inkingi ni ibikoresho bishya byihariye bya Foregene, bishobora guhuza neza na ADN, kandi bigakuraho cyane RNA, poroteyine zanduye, ion hamwe n’ibindi binyabuzima mu ngirabuzimafatizo.ADN ya 5-80μg yo mu rwego rwo hejuru irashobora kwezwa kuva 10-50mg.

Ibisobanuro

*: Buffer L2 na Buffer PW zirimo umunyu ushimishije.Nyamuneka kwambara uturindantoki kandi ufate ingamba zo kubarinda mugihe ukora.

Ibiranga ibyiza

■ Nta kwanduza RNase: Inkingi ya ADN yonyine yatanzwe mu gikoresho ituma bishoboka kuvana RNA muri ADN ya genomic nta RNase yongeyeho mu gihe cy’igerageza, bityo bikabuza laboratoire kwanduzwa na RNase idasanzwe.

Speed ​​Umuvuduko wihuse: Foregene Protease ifite ibikorwa byinshi kuruta protease isa kandi igogora ingero za tissue byihuse.

Byoroshye: igikorwa cyo gukuramo ADN genomic kirashobora kurangira muminota 50.

■ Byoroshye: Centrifugation ikorwa mubushyuhe bwicyumba, nta 4 cent ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol irakenewe.

Umutekano: nta reagent ngenga isabwa.

Quality Ubwiza buhanitse: ADN yakuwe muri ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, birashobora kuzuza ibisabwa mubushakashatsi butandukanye.

Porogaramu

Irakwiriye gukuramo no kweza ADN genomic muri selile zitandukanye zumuco mushya cyangwa zikonje hamwe ninyama zinyamaswa.

Urujya n'uruza rw'akazi

Igishushanyo

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) na 2-8 ℃ mugihe kirekire.

Kugira ngo duhuze ibyifuzo byabakiriya byateganijwe, ubu dufite abakozi bacu bakomeye kugirango batange ubufasha bukomeye burimo ibicuruzwa, kugurisha, gutegura, gukora, kugenzura ubuziranenge bwo hejuru, gupakira, ububiko hamwe nibikoresho byo muruganda byakozwe bishyushye-kugurisha Magnetic Bead Nucleic Acide Gukuramo ibikoresho Rna Kwigunga ADN Isukura PCR Ikizamini, "Hindura kuri byinshi byiza!"ni intero yacu, bisobanura ngo “Isi nziza iri imbere yacu, reka rero tuyikunde!”Hindura kuri byinshi byiza!Mwese muriteguye?
Uruganda rwakoze-kugurishaUbushinwa Magnetic Isaro hamwe nigitabo cyo gusuzuma, Ubunararibonye bwacu butugira ingenzi mumaso yabakiriya bacu.Ubwiza bwacu buvuga ubwabwo imitungo nkiyi idahindagurika, isuka cyangwa isenyuka, ibyo rero nibyo abakiriya bacu bazahora bizeye mugihe batanga itegeko.