Turashobora guhora duhaza abakiriya bacu bubashywe nibyiza byacu byiza byo hejuru, igiciro cyiza hamwe ninkunga nziza kuberako twabaye inzobere yinyongera kandi dukora cyane kandi tukabikora muburyo buhendutse kubwiza buhebuje Ubushinwa Bwiza cyane kandi buhamye Lyophilized Dspathtm 4X Intambwe imwe Multiplex Master Mix, Menya neza ko wumva rwose ufite umudendezo wo kutuvugisha kumuryango.ndizera ko tugiye gusangira ubucuruzi bwiza bwubucuruzi bwiza nabacuruzi bacu bose.
Turashobora guhora duhaza abakiriya bacu bubashywe nibyiza byacu byiza, igiciro cyiza hamwe ninkunga nziza kuberako twabaye inzobere yinyongera kandi dukora cyane kandi tubikora muburyo buhendutse kuriUbushinwa Taq ADN Polymerase, Qpcr, Isosiyete yacu ikomeje gukorera abakiriya bafite ubuziranenge bwo hejuru, igiciro cyo gupiganwa no gutanga ku gihe.Twishimiye byimazeyo inshuti ziturutse impande zose z'isi gufatanya natwe no kwagura ibikorwa byacu.Niba ushimishijwe nibicuruzwa byacu, ugomba kumva utwandikira.Twifuzaga kuguha andi makuru.

Ibisobanuro

5ml, 10ml, 40ml

PCR Intwari (Hamwe Irangi) Ibikoresho byo gukuramo birimo Foregene idasanzwe idasanzwe ya Taq - Foregene Taq Intwari, optime ya DNTPs, MgCl2, buffer reaction, PCR itera imbaraga, optimizer na stabilisateur.Sisitemu ya 2 × PCR HeroTM ivanze (irangi) ifite kwihanganira cyane inhibitori za PCR kuruta sisitemu isanzwe ya PCR ivanze, kandi irashobora guhangana byoroshye na PCR yogukwirakwiza inyandikorugero zitandukanye.Sisitemu idasanzwe ya reaction hamwe nubushobozi buhanitse Taq Intwari ituma reaction ya PCR igira ubushobozi bwo kongera amplification, umwihariko hamwe na sensitivite.

Ibigize ibikoresho

Ibiranga ibyiza

Ubudahemuka buhebuje: inshuro 6 z'umusemburo wa Taq usanzwe;

Speed ​​Umuvuduko wihuse wihuta: umuvuduko wa amplification ni 5-10sec / kb, wikubye inshuro 6-12 ugereranije na Taq enzyme isanzwe;

Ibindi byitegererezo byo guhuza n'imiterere: Irashobora kwagura neza inyandikorugero zitandukanye za ADN hamwe na hejuruIbirimo GC;

Efficiency Gukora neza cyane: kugabanuka kwinshi kurenza imisemburo ya Taq isanzwe;

Erance Kwihanganira ibidukikije birakomeye: bishyirwa kuri 37 ° C icyumweru, bikomeza ibikorwa birenga 90%;

Ifite ibikorwa 5 ′ → 3 ′ ibikorwa bya ADN polymerase na 5 ′ → 3 ′ ibikorwa bya exonuclease, nta 3 ′ → 5 ′ ibikorwa bya exonuclease.

Kit Ibikoresho byabanjirijwe kuvangwa na Electrophoresis Loading Buffer, PCR imaze kurangira, icyitegererezo gishobora gutwarwa na electrophorei;imikorere iroroshye kandi yoroshye.

Porogaramu

Kwiyongera kwa PCR ibice bya ADN, ibimenyetso bya ADN, uko ADN ikurikirana, PCR wongeyeho umurizo

Ububiko hamwe nubuzima bwa Shelf

Irashobora kubikwa igihe kirekire kuri -20 ° C;kubikoresha kenshi, birashobora kubikwa kuri 4 ° C mugihe gito. Turashobora guhora duhaza abakiriya bacu bubashywe hamwe nibyiza byacu byiza, igiciro cyiza hamwe ninkunga nziza kuberako twabaye inzobere yinyongera kandi dukora cyane kandi tukabikora muburyo buhendutse kubwiza buhebuje Ubushinwa Bwiza cyane kandi buhamye Lyophilized Dspathtm 4X Intambwe imwe Multiplex Master mix to make.ndizera ko tugiye gusangira ubucuruzi bwiza bwubucuruzi bwiza nabacuruzi bacu bose.
Ubwiza buhebujeUbushinwa Taq ADN Polymerase, Qpcr, Isosiyete yacu ikomeje gukorera abakiriya bafite ubuziranenge bwo hejuru, igiciro cyo gupiganwa no gutanga ku gihe.Twishimiye byimazeyo inshuti ziturutse impande zose z'isi gufatanya natwe no kwagura ibikorwa byacu.Niba ushimishijwe nibicuruzwa byacu, ugomba kumva utwandikira.Twifuzaga kuguha andi makuru.