Turakomeza gutera imbere no gutunganya ibintu byacu no gusana.Mugihe kimwe, dukora cyane kugirango dukore ubushakashatsi niterambere mugiciro cyo kugabanura Ubushinwa Bioer Bsc70 Simplyp Animal Pathogens ADN / Rna Extraction Kit Manufacturer Producer, Twishimiye cyane ibibazo byose byabajijwe kuva murugo no mumahanga kugirango dufatanye natwe, kandi dutegereje ubutumwa bwawe.
Turakomeza gutera imbere no gutunganya ibintu byacu no gusana.Mugihe kimwe, dukora cyane kugirango dukore ubushakashatsi niterambereUbushinwa ADN / Rna, Amasaro ya Magnetique, Dushyigikiwe nabanyamwuga bacu b'inararibonye, ​​dukora kandi tugatanga ibicuruzwa byiza.Ibi ni byiza kugeragezwa mubihe bitandukanye kugirango tumenye neza ko intera itagira inenge igezwa kubakiriya, natwe turateganya umurongo nkuko tubikeneye kugira abakiriya kugirango babone ibyo abakiriya bakeneye.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Ibigize ibikoresho

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Turakomeza gutera imbere no gutunganya ibintu byacu no gusana.Mugihe kimwe, dukora cyane kugirango dukore ubushakashatsi niterambere mugiciro cyo kugabanura Ubushinwa Bioer Bsc70 Simplyp Animal Pathogens ADN / Rna Extraction Kit Manufacturer Producer, Twishimiye cyane ibibazo byose byabajijwe kuva murugo no mumahanga kugirango dufatanye natwe, kandi dutegereje ubutumwa bwawe.
IgiciroUbushinwa ADN / Rna, Amasaro ya Magnetique, Dushyigikiwe nabanyamwuga bacu b'inararibonye, ​​dukora kandi tugatanga ibicuruzwa byiza.Ibi ni byiza kugeragezwa mubihe bitandukanye kugirango tumenye neza ko intera itagira inenge igezwa kubakiriya, natwe turateganya umurongo nkuko tubikeneye kugira abakiriya kugirango babone ibyo abakiriya bakeneye.