Ubushinwa Bwiza RNA bufite ireme Uruganda nuwitanga |Foregene

Ubwiza bwa RNA

Ibisobanuro:

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi nziza-yuzuye ya RNA ivuye mu ngirabuzimafatizo zifite 96, 24, 12, na 6-nziza.Igikoresho gitanga ADN isukura neza, ishobora gutandukanya byoroshye na lysate ndengakamere, guhuza no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe;Inkingi ya RNA yonyine irashobora guhuza neza RNA hamwe na formula idasanzwe.Umubare munini wintangarugero urashobora gutunganyirizwa icyarimwe.

Sisitemu yose ni RNase-Yubusa, kugirango RNA isukuye ntisuzugurwe;Buffer RW1, Buffer RW2 sisitemu yo gukaraba ya garanti ya RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda w’ibinyabuzima.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Twifashishije porogaramu yuzuye ya siyansi yuzuye yo gucunga neza, ireme ryiza kandi ryizera cyane, turabona izina ryiza kandi twigaruriye ingandaUbwiza bwa RNA, Igitekerezo cyacu ni "Ibiciro bifatika, igihe cyumusaruro wubukungu na serivisi nziza cyane" Turizera ko tuzafatanya nabaguzi benshi mugutezimbere no kunguka.
Twifashishije porogaramu yuzuye ya siyansi yuzuye yo gucunga neza, ireme ryiza kandi ryizera cyane, turabona izina ryiza kandi twigaruriye ingandaUbwiza bwa RNA, Isosiyete yacu ifata "ibiciro byumvikana, ubuziranenge bwo hejuru, igihe cyiza cyo gukora neza na serivisi nziza nyuma yo kugurisha" nkibisobanuro byacu.Turizera gufatanya nabakiriya benshi mugutezimbere hamwe ninyungu mugihe kizaza.Murakaza neza kutwandikira.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Ibigize ibikoresho

Buffer cRL1, Buffer cRL2

Buffer RW1, Buffer RW2

Ubusa ddH₂O, Inkingi-yoza

RNA-Inkingi gusa

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

selile yose RNA

Igishushanyo

Akagari Igiteranyo Cyuzuye RNA Igikoresho Cyakazi Flow1

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) na 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (itabishaka) .Gukoresha gahunda yuzuye yubuhanga buhanitse bwo gucunga neza, ubuziranenge bwo mu rwego rwo hejuru kandi kwizera gukomeye, twamamaye cyane kandi dukora inganda zinganda zujuje ubuziranenge Ubushinwa Longyu Gutanga ubuziranenge bwizewe hamwe nubucuruzi bwiza kandi twizera cyane kubiciro byingirakamaro hamwe nubucuruzi bwiza.
Ubwiza buhanitse kubushinwa Nomilin, Gukuramo Solvent, Isosiyete yacu ibona "ibiciro byumvikana, ubuziranenge bwiza, igihe cyiza cyo gukora neza na serivisi nziza nyuma yo kugurisha" nkibisobanuro byacu.Turizera gufatanya nabakiriya benshi mugutezimbere hamwe ninyungu mugihe kizaza.Murakaza neza kutwandikira.

Mbere: Igiciro Cyinshi Ubushinwa Ubushinwa Bwiza Bwiza 1% 1-Deoxynojirimycin Mulberry Amababi

Ibikurikira: gukuramo RNA yose

Andika ubutumwa bwawe hano hanyuma utwohereze