Ubushinwa Ubushinwa Bitanga Ubushinwa Uruganda Magnetic Isaro Acide Nucleic Test Kit Rt-PCR Igikoresho nuwitanga |Foregene

Ubushinwa butanga Ubushinwa Uruganda Magnetic Isaro Acide Nucleic Test Kit Rt-PCR Kit

Ubushinwa Utanga Ubushinwa Uruganda Magnetic Isaro Acide Nucleic Test Kit Rt-PCR Kit Ikiranga Ishusho

Ibisobanuro:

Iki gikoresho gikoresha sisitemu yihariye ya lysis kugirango irekure vuba ADN genomic muri zebrafish nizindi nyama zamafi yamazi meza, imirizo yumurizo cyangwa amagi y amafi byerekana PCR, bityo rero birakwiriye cyane cyane kwipimisha geneti nini.Igikorwa cyo kurekura ADN genomic muri buffer ya lysis kirangiye muminota 10-30 kuri 65 ° C.Ntayindi nzira nka protein na RNA ikuraho, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.

2 × PCR EasyTM ivanze (UNG) ikoresha dUTP aho gukoresha dTTP hashingiwe kuri 2 × PCR EasyTM ivanze, ikongeramo enzyme ya UNG (Uracil-N-glycosylase) ishobora gutesha agaciro inyandikorugero irimo dUTP icyarimwe.Mbere ya PCR reaction, enzyme ya UNG ikoreshwa mugutesha agaciro ibicuruzwa bya PCR birimo uracil.Enzyme ya UNG ntacyo izagira ku gishushanyo kitarimo uracil, bityo kikaba cyemeza umwihariko n'ukuri kwa amplification no gukumira ko hashobora kubaho ibizamini binini.Kwanduza ibicuruzwa bya PCR byabaye.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Dufite itsinda ryiza cyane kugirango dukemure ibibazo byabakiriya.Intego yacu ni "100% kunyurwa byabakiriya kubwiza bwibicuruzwa byacu, igiciro & serivisi yacu" kandi tunezezwa neza nabakiriya.Hamwe ninganda nyinshi, turashobora gutanga ibicuruzwa byinshi mubushinwa butanga Ubushinwa Uruganda Magnetic Bead Acide Nucleic Test Kit Rt-PCR Kit, Uruganda rwacu rwahise rukura mubunini no kumenyekana kubera ubwitange bwuzuye mubikorwa byiza byo hejuru, igiciro kinini cyibisubizo na serivisi nziza zabakiriya.
Dufite itsinda ryiza cyane kugirango dukemure ibibazo byabakiriya.Intego yacu ni "100% kunyurwa byabakiriya kubwiza bwibicuruzwa byacu, igiciro & serivisi yacu" kandi tunezezwa neza nabakiriya.Hamwe ninganda nyinshi, turashobora gutanga intera nini yaIgikoresho cyo gukuramo Ubushinwa, Gukuramo Acide Nucleic, Isosiyete yacu ihora yibanda kumajyambere yisoko mpuzamahanga.Ubu dufite abakiriya benshi mu Burusiya, mu bihugu by’Uburayi, Amerika, ibihugu byo mu burasirazuba bwo hagati no mu bihugu bya Afurika.Buri gihe dukurikiza ubwo bwiza ni umusingi mugihe serivisi ari garanti yo guhura nabakiriya bose.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.	Buffer FP
	Foregene Protease
	6 × ADN Yipakurura Buffer
Igice cya II	2 × PCR Biroroshye^TMKuvanga (UNG)
Amabwiriza

Ibiranga ibyiza

■ Nta bisabwa bitwara igihe kandi bihenze bya ADN bisabwa.

Require Icyitegererezo gikenewe ni gito, nkibishobora 1mg amafi ya caudal fin cyangwa amagi 10 y amafi arashobora gupimwa.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu yo kurwanya umwanda PCR 2 × PCR EasyTM Mix (UNG), ishobora gukuraho neza umwanda uterwa n’ibicuruzwa bya PCR kandi ikemeza umwihariko n’ukuri kwa amplification.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ibipimo by'ibikoresho

Gushyira mu bikorwa: Amafi ya Zebra nandi mafi yamazi meza.

ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.

Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.

Buffer FP reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.

v Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.

vReagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.

Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.

vReagent 2 × PCR EasyTM ivanze (UNG), niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ℃ kubikwa igihe gito (koresha muminsi 10).

Dufite itsinda ryiza cyane kugirango dukemure ibibazo byabakiriya.Intego yacu ni "100% kunyurwa byabakiriya kubwiza bwibicuruzwa byacu, igiciro & serivisi yacu" kandi tunezezwa neza nabakiriya.Hamwe ninganda nyinshi, turashobora gutanga ibicuruzwa byinshi mubushinwa butanga Ubushinwa Uruganda Magnetic Bead Acide Nucleic Test Kit Rt-PCR Kit, Uruganda rwacu rwahise rukura mubunini no kumenyekana kubera ubwitange bwuzuye mubikorwa byiza byo hejuru, igiciro kinini cyibisubizo na serivisi nziza zabakiriya.
UbushinwaIgikoresho cyo gukuramo Ubushinwa, Gukuramo Acide Nucleic, Isosiyete yacu ihora yibanda kumajyambere yisoko mpuzamahanga.Ubu dufite abakiriya benshi mu Burusiya, mu bihugu by’Uburayi, Amerika, ibihugu byo mu burasirazuba bwo hagati no mu bihugu bya Afurika.Buri gihe dukurikiza ubwo bwiza ni umusingi mugihe serivisi ari garanti yo guhura nabakiriya bose.

Mbere: Abashinwa babigize umwuga Ubushinwa Nucleic Acide Gukuramo Reagent (Serum / Plasma na Puncture solution), Ibikoresho byo Gukuramo Rna

Ibikurikira: Igiciro cyuruganda Ubushinwa Izina rusange: Gukuramo Acide Nucleic

Andika ubutumwa bwawe hano hanyuma utwohereze