Urutonde ruhendutse kubushinwa RNA Kwigunga hamwe na CE
Turashimangira ku gitekerezo cyo gukura kwa 'High excellent, Performance, Sincerity and Down-to-work work work' kugirango tuguhe hamwe nisosiyete nini yo gutunganya ibiciro bihendutse kubushinwa RNA Isolation Kit hamwe na CE, Twishimiye iperereza ryanyu kandi ni icyubahiro gukorana ninshuti zose kwisi.
Turashimangira kubijyanye nigitekerezo cyo gukura kw '' Byiza cyane, Imikorere, Ubunyangamugayo hamwe nuburyo bwo gukora hasi 'kugirango tuguhe hamwe nisosiyete ikomeye yo gutunganyaUbushinwa RNA Ikuramo, Rna Kwigunga, Ibikorwa remezo bikomeye nubushake bwumuryango uwo ariwo wose.Twashyigikiwe nibikoresho remezo bikomeye bidushoboza gukora, kubika, kugenzura ubuziranenge no kohereza ibicuruzwa nibisubizo kwisi yose.Kugirango dukomeze akazi neza, twagabanije ibikorwa remezo mubice byinshi.Aya mashami yose arakora nibikoresho bigezweho, imashini nibikoresho bigezweho.Kubera iyo mpamvu, turashoboye gukora umusaruro mwinshi tutabangamiye ubuziranenge.
Kits Ibisobanuro
50 Imyiteguro, 200 Imyiteguro
Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.
Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.
Ibigize ibikoresho:
| Buffer RL1, Buffer RL2 |
| Buffer RW1, Buffer RW2 |
| RNase-Yubusa ddH2O, Inkingi-Yeza |
| RNA-Inkingi gusa |
Ibiranga ibyiza
■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1
Porogaramu
Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.
Ibipimo byibicuruzwa
Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 106
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl
Urujya n'uruza rw'akazi
Igishushanyo
Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar
Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima
Ububiko nubuzima bwiza
Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).
Turashimangira ku gitekerezo cyo gukura kwa 'High excellent, Performance, Sincerity and Down-to-work work work' kugira ngo tuguhe hamwe n’isosiyete nini yo gutunganya ibiciro bihendutse ku Bushinwa 96t ADN Isolation Kit hamwe na CE na FDA, Twishimiye iperereza ryanyu kandi ni icyubahiro gukorana n'inshuti zose ku isi.
Urutonde ruhendutse kurutonde rwubushinwa ADN ikuramo ibikoresho, ibikoresho bya ADN byo kwigunga, ibikorwa remezo bikomeye nicyo cyifuzo cyumuryango uwo ariwo wose.Twashyigikiwe nibikoresho remezo bikomeye bidushoboza gukora, kubika, kugenzura ubuziranenge no kohereza ibicuruzwa nibisubizo kwisi yose.Kugirango dukomeze akazi neza, twagabanije ibikorwa remezo mubice byinshi.Aya mashami yose arakora nibikoresho bigezweho, imashini nibikoresho bigezweho.Kubera iyo mpamvu, turashoboye gukora umusaruro mwinshi tutabangamiye ubuziranenge.
