Dutsimbaraye ku myizerere ya "Gukora ibintu hejuru yurwego no gushiraho inshuti nabantu muri iki gihe ku isi yose", mubisanzwe dushyira inyungu zabaguzi kumwanya wambere wo gutanga ODM Ubushinwa CE FDA IhuzaGukuramo Acide NucleicReagent Kit 96 Ibizamini, Iyo uhiga rimwe na rimwe ubuziranenge bwo hejuru ku giciro cyiza no gutanga ku gihe.Kora natwe.
Kwizirika ku myizerere ya "Kurema ibintu hejuru yurwego no gushiraho inshuti hamwe nabantu muri iki gihe baturutse kwisi yose", mubisanzwe dushyira inyungu zabaguzi kumwanya wa mbere kuriUbushinwa Gukuramo Acide Nucleic Reagent, Gukuramo Acide Nucleic, Ubu dufite imirimo irenga 100 muruganda, kandi dufite itsinda ryabasore 15 bakorera abakiriya bacu mbere na nyuma yo kugurisha.Ubwiza bwiza nicyo kintu cyingenzi kugirango sosiyete ihagarare kubandi bahanganye.Kubona ni Kwizera, ushaka amakuru menshi?Gerageza gusa kubicuruzwa byayo!

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi nziza-yuzuye ya RNA ivuye mu ngirabuzimafatizo zifite 96, 24, 12, na 6-nziza.

Igikoresho gitanga ADN isukura neza, ishobora gutandukanya byoroshye na lysate ndengakamere, guhuza no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe.

Inkingi ya RNA yonyine irashobora guhuza neza RNA hamwe na formula idasanzwe.Umubare munini wintangarugero urashobora gutunganyirizwa icyarimwe.

Ibigize ibikoresho

* Nyamuneka wambare uturindantoki kandi ufate ingamba zo kubarinda mugihe cyo kubaga kuko Buffer cRL1 na Buffer RW1 zirimo umunyu wa chaotropique.

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

Igishushanyo

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka).

Dutsimbaraye ku myizerere ya "Kurema ibintu biri hejuru yurwego no gushiraho inshuti hamwe nabantu muri iki gihe baturutse kwisi yose", mubisanzwe dushyira inyungu zabaguzi kumwanya wambere mugutanga ODM Ubushinwa CE FDA Ihuza Nucleic Acide Gukuramo Reagent Kit 96 Ibizamini, Iyo uhiga rimwe na rimwe ubuziranenge bwo hejuru mugiciro cyiza kandi gitangwa mugihe gikwiye.Kora natwe.
Tanga ODMUbushinwa Gukuramo Acide Nucleic Reagent, Gukuramo Acide Nucleic, Ubu dufite imirimo irenga 100 muruganda, kandi dufite itsinda ryabasore 15 bakorera abakiriya bacu mbere na nyuma yo kugurisha.Ubwiza bwiza nicyo kintu cyingenzi kugirango sosiyete ihagarare kubandi bahanganye.Kubona ni Kwizera, ushaka amakuru menshi?Gerageza gusa kubicuruzwa byayo!