Twabaye inararibonye mu gukora.Gutsindira ibyinshi mubyemezo byawe byingenzi byamasoko yayo yo kugura super kugura Ubushinwa Real-Time PCR, Niba ushishikajwe nibicuruzwa hafi ya byose, wibuke kumva rwose ufite umudendezo rwose kugirango utumenyeshe amakuru yandi makuru cyangwa wizere neza ko utugezaho imeri neza, tuzagusubiza mumasaha 24 gusa kimwe nibisobanuro byiza bigiye gutangwa.
Twabaye inararibonye mu gukora.Gutsindira ibyinshi mubyemezo byawe byingenzi byisoko ryayoUbushinwa PCR, Igihe nyacyo Pcr, Hamwe n'umwuka wo gushishikarira "gukora neza, korohereza, gushyira mu bikorwa no guhanga udushya", kandi dukurikije ubwo buryo bwo gutanga serivisi nziza "nziza, ariko igiciro cyiza," na "inguzanyo ku isi", duharanira gufatanya n’amasosiyete akora ibice by’imodoka ku isi hose kugira ngo habeho ubufatanye bwunguka.

Ibisobanuro

Iki gikoresho gikoresha sisitemu idasanzwe ya lysis buffer ishobora kurekura byihuse RNA mumikorere yimikorere yimikorere ya RT-qPCR, bityo bikuraho inzira yo kweza RNA itwara igihe kandi ikora cyane.Inyandikorugero ya RNA irashobora kuboneka muminota 7 gusa.5 × Direct RT ivanze na 2 × Imiyoboro ya qPCR ivanze-SYBR itangwa nigitabo irashobora kwihuta kandi neza kubona ibisubizo nyabyo-byuzuye bya PCR.

5 × Direct RT ivanze na 2 × Direct qPCR ivanze-SYBR ifite kwihanganira imbaraga za inhibitor, kandi lysate yintangarugero irashobora gukoreshwa nkicyitegererezo cya RT-qPCR itaziguye.Iki gikoresho kirimo RNA idasanzwe-yuzuye ya Foregene reverse transcriptase, hamwe na polymerase ya D-Taq ishyushye, DNTPs, MgCl₂, reaction ya reaction, optimizer ya PCR na stabilisateur.

Ibisobanuro

200 × 20μl Rxns, 1000 × 20μl Rxns

Ibigize ibikoresho

Ibiranga ibyiza

■ Byoroshye kandi byiza: hamwe na tekinoroji ya Direct Direct RT, ingero za RNA zirashobora kuboneka muminota 7 gusa.

Icyitegererezo gikenewe ni gito, nkuko selile 10 zishobora kugeragezwa.

Thr Kwinjiza cyane: irashobora kumenya vuba RNA mu ngirabuzimafatizo zifite 384, 96, 24, 12, 6-amasahani meza.

Er ADN Eraser irashobora gukuraho vuba genome zasohotse, bikagabanya cyane ingaruka kubisubizo byakurikiyeho.

System Optimized RT na qPCR sisitemu ituma intambwe ebyiri RT-PCR ihinduranya transcript neza kandi PCR ikarushaho kuba nziza, kandi ikarwanya RT-qPCR inhibitor.

Porogaramu

Igipimo cyo gushyira mu bikorwa: ingirabuzimafatizo.

- RNA yarekuwe na sample lysis: ikoreshwa gusa kuri RT-qPCR inyandikorugero yiki gitabo.

- Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: gusesengura imvugo ya gene, kugenzura ingaruka zo gucecekesha gene ya siRNA, gusuzuma ibiyobyabwenge, nibindi.

Igishushanyo

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 4 ℃;Igice cya II kigomba kubikwa kuri -20 ℃.

Foregene Protease Plus II igomba kubikwa kuri 4 ℃, ntugahagarike kuri -20 ℃.

Reagent 2 × Direct qPCR Ivanga-SYBR igomba kubikwa kuri -20 ℃ mu mwijima;niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ℃ kubikwa igihe gito (koresha muminsi 10) .Twabaye inararibonye mubukora.Gutsindira ibyinshi mubyemezo byawe byingenzi byisoko ryayo ryo kugura super kugura Ubushinwa Q160 / Q320 Real-Time PCR, Niba ushishikajwe nibicuruzwa hafi ya byose, wibuke kumva rwose ufite umudendezo rwose kugirango utumenyeshe amakuru yandi cyangwa wizere neza ko utugezaho imeri neza, tuzagusubiza mumasaha 24 gusa kimwe nibisobanuro byiza bigiye gutangwa.
Kugura ByinshiUbushinwa PCR, Igihe nyacyo PCR, Hamwe n'umwuka wo guharanira "gukora neza, korohereza, gushyira mu bikorwa no guhanga udushya", kandi dukurikije ubwo buryo bwo gutanga serivisi nziza "igiciro cyiza ariko cyiza," na "inguzanyo ku isi", duharanira gufatanya n’amasosiyete akora ibice by’imodoka ku isi hose kugira ngo dukore ubufatanye bwunguka.