Dufite ibikoresho bigezweho.Ibicuruzwa byacu byoherezwa muri Amerika, Ubwongereza nibindi, byishimira izina ryiza mubakiriya kubiciro byumvikana kubushinwa Rapid na High Yeild Virus Total Rna Isolation Extraction Purification Kit Nucleic Acide Detection Kit, Tugiye guhora twihatira kongera uruganda rwacu no gutanga ibicuruzwa byiza bifite igiciro cyinshi.Ikibazo cyangwa igitekerezo icyo aricyo cyose kirashimirwa cyane.Wibuke kudufata mu bwisanzure.
Dufite ibikoresho bigezweho.Ibicuruzwa byacu byoherezwa muri Amerika, Ubwongereza nibindi, bikagira izina ryiza mubakiriya kuriAutomatic Nucleic Acide, Ubushinwa Universal Rna ADN Reagents, Twite cyane kuri serivisi zabakiriya, kandi dukunda buri mukiriya.Ubu twakomeje kugira izina rikomeye mu nganda imyaka myinshi.Twabaye inyangamugayo kandi dukora kugirango twubake umubano muremure nabakiriya bacu.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nini kandi nziza-yuzuye ya RNA ivuye mu ngirabuzimafatizo zifite 96, 24, 12, na 6-nziza.

Igikoresho gitanga ADN isukura neza, ishobora gutandukanya byoroshye na lysate ndengakamere, guhuza no gukuraho ADN genomic.Igikorwa kiroroshye kandi gitwara igihe.

Inkingi ya RNA yonyine irashobora guhuza neza RNA hamwe na formula idasanzwe.Umubare munini wintangarugero urashobora gutunganyirizwa icyarimwe.

Ibigize ibikoresho

* Nyamuneka wambare uturindantoki kandi ufate ingamba zo kubarinda mugihe cyo kubaga kuko Buffer cRL1 na Buffer RW1 zirimo umunyu wa chaotropique.

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

Igishushanyo

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka). Dufite ibikoresho bigezweho.Ibicuruzwa byacu byoherezwa muri Amerika, Ubwongereza nibindi, byishimira izina ryiza mubakiriya kubiciro byumvikana kubushinwa Rapid na High Yeild Virus Total Rna Isolation Extraction Purification Kit Nucleic Acide Detection Kit, Tugiye guhora twihatira kongera uruganda rwacu no gutanga ibicuruzwa byiza bifite igiciro cyinshi.Ikibazo cyangwa igitekerezo icyo aricyo cyose kirashimirwa cyane.Wibuke kudufata mu bwisanzure.
Igiciro cyumvikana kuriUbushinwa Universal Rna ADN Reagents, Automatic Nucleic Acide, Twite cyane kuri serivisi zabakiriya, kandi dukunda buri mukiriya.Ubu twakomeje kugira izina rikomeye mu nganda imyaka myinshi.Twabaye inyangamugayo kandi dukora kugirango twubake umubano muremure nabakiriya bacu.