Amagambo yihuse kandi meza, abajyanama bamenyeshejwe kugirango bagufashe guhitamo ibicuruzwa byiza bihuye nibisabwa byose, igihe gito cyo gukora, gucunga neza ubuziranenge bwo hejuru hamwe na serivisi zidasanzwe zo kwishyura no kohereza ibicuruzwa kubiciro byavuzwe kubushinwaKanda-It Edu-647Akagari ko gukwirakwiza selile, uruganda rwambere, twunvikana.Isosiyete yinyongera, ikizere kiragerayo.Firime yacu mubisanzwe kuri serivisi yawe igihe icyo aricyo cyose.
Amagambo yihuse kandi meza, abajyanama bamenyeshejwe kugirango bagufashe guhitamo ibicuruzwa byiza bihuye nibisabwa byose, igihe gito cyo gukora, gucunga neza ubuziranenge bwo hejuru hamwe na serivisi zidasanzwe zo kwishyura no kohereza ibintuIgikoresho cyo gukwirakwiza Ubushinwa, Kanda-It Edu-647, Ibicuruzwa byacu bizwi cyane kandi byizewe nabakoresha kandi birashobora guhora bihinduka mubyifuzo byubukungu n’imibereho.Twakiriye neza abakiriya bashya nabakera baturutse imihanda yose kugirango batubwire umubano wubucuruzi hamwe no gutsinda!

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo mer- mercaptoethanol (bidashoboka) .Ijambo ryihuse kandi ryiza, ryamenyeshejwe abajyanama kugufasha guhitamo ibicuruzwa byiza bikwiranye nibisabwa byose, igihe gito cyo gukora, gucunga neza ubuziranenge hamwe na serivisi zidasanzwe zo kwishyura no kohereza ibicuruzwa kubiciro byavuzwe mubushinwa Kanda-It Edu-647 Gukwirakwiza Akagari, Dutangije Ikibanza,Isosiyete yinyongera, ikizere kiragerayo.Firime yacu mubisanzwe kuri serivisi yawe igihe icyo aricyo cyose.
Igiciro cyatanzwe kuriIgikoresho cyo gukwirakwiza Ubushinwa, Kanda-It Edu-647, Ibicuruzwa byacu birazwi cyane kandi byizewe nabakoresha kandi birashobora guhora bihinduka mubyifuzo byubukungu n’imibereho.Twakiriye neza abakiriya bashya nabakera baturutse imihanda yose kugirango batubwire umubano wubucuruzi hamwe no gutsinda!