Ubwiza Bwambere, kandi Umukiriya wikirenga nuyobozo wo gutanga serivise nziza kubakiriya bacu.Muri iki gihe, turagerageza uko dushoboye kugira ngo tube umwe mu bohereza ibicuruzwa byiza mu mahanga mu rwego rwacu kugira ngo duhuze abakiriya bakeneye cyane Igishushanyo mbonera cy’Ubushinwa 96t Automatic ADN RnaAcide Nucleic AcideIgisubizo hamwe na CE na FDA, Twishimiye ibyiringiro byo gukora ubucuruzi buciriritse hamwe nawe kandi twizera ko tuzishimira guhuza nibindi bicuruzwa byacu.
Ubwiza Bwa mbere, kandi Umukiriya w'Ikirenga ni umurongo ngenderwaho wo gutanga serivisi nziza kubakiriya bacu.Muri iki gihe, turagerageza uko dushoboye kugira ngo tube umwe mu bohereza ibicuruzwa byiza mu mahanga mu rwego rwacu kugira ngo duhuze abakiriya bakeneye cyane.Ubushinwa Nucleic Acide Isolation Solution, Acide Nucleic Acide, Kubantu bose bashishikajwe nikintu icyo aricyo cyose nyuma yo kureba urutonde rwibicuruzwa byacu, ugomba rwose kumva ufite umudendezo rwose kugirango utumenyeshe ibibazo.Urashobora kutwoherereza imeri hanyuma ukatwandikira kugirango tugishe inama kandi tuzagusubiza vuba bishoboka.Niba byoroshye, urashobora kumenya aderesi yacu kurubuga rwacu hanyuma ukaza mubucuruzi bwacu kubindi bisobanuro byinshi byibicuruzwa byacu wenyine.Twama twiteguye kubaka umubano mugari kandi uhamye wubufatanye nabakiriya bose bashoboka mubice bifitanye isano.

Ibisobanuro

50 Itegure, kugirango genomic ADN isukure mumaraso yose ukoresheje <1ml maraso

Buffer DC: Lyses selile zitukura mumaraso.

Buffer BL1: Tanga icyitegererezo cyimyunyu ngugu.

Foregene Protease Yongeyeho: Enzymatique igogora ingero zamaraso mubidukikije bya Buffer BL1.

Buffer BL2: Kora Foregene Protease Plus kandi utange ibidukikije byapakira ADN.

Buffer PW: Kuraho umwanda nka proteyine na RNA muri ADN.

Buffer WB1: Kuraho ion zisigaye muri ADN.

Buffer EB: Hindura ADN kumurongo wo kweza.

ADN-Inkingi Yonyine: Byumwihariko byamamaza ADN genomic muri lysate.

Ibigize ibikoresho

Ibiranga ibyiza

-Nta kwanduza RNase: Inkingi ya ADN-Yonyine yatanzwe nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utiriwe wongera RNase mugihe cyigeragezo, ukirinda laboratoire kwanduzwa na RNase idasanzwe.

-Umuvuduko wihuse: Foregene Protease Plus ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue byihuse;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 40.

-Icyoroshye: Centrifugation ikorwa mubushyuhe bwicyumba, 4 ° C centrifugation cyangwa Ethanol imvura ya ADN ntabwo isabwa.

-Umutekano: Nta gukuramo organic reagent ikoreshwa.

-Ubuziranenge bwo hejuru: Ibice bya ADN byakuweho ni binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.

Porogaramu

Birakwiriye gukuramo no kweza ADN genomic mumaraso mashya cyangwa akonje.

Urupapuro rw'akazi

Ububiko hamwe nubuzima bwa Shelf

Iki gikoresho gishobora kubikwa amezi 12 mugihe cyumye mubushyuhe bwicyumba (15-25 ° C);niba ikeneye kubikwa mugihe kirekire, irashobora kubikwa kuri 2-8 ° C.

Icyitonderwa: Niba bibitswe ku bushyuhe buke, igisubizo gikunda kugwa.Mbere yo gukoresha, menya neza ko ushira igisubizo mubikoresho mubushyuhe bwicyumba mugihe runaka.Nibiba ngombwa, shyushya mu bwogero bwa 37 ° C bwamazi muminota 10 kugirango ushongeshe imvura, hanyuma ubivange mbere yo kubikoresha.

Igisubizo cya Foregene Protease Plus gifite formula idasanzwe, ikora iyo ibitswe mubushyuhe bwicyumba igihe kinini (amezi 3);bizarushaho gukora neza kandi bihamye mugihe bibitswe kuri 4 ° C, birasabwa rero kubibika kuri 4 ° C, wibuke kutabika kuri -20 ° C.Ubwiza bwa mbere, kandi Umukiriya wikirenga ni umurongo ngenderwaho wo gutanga serivise nziza kubakiriya bacu.Mu minsi, turagerageza gukora ibishoboka byose kugirango tube umwe mubatumiza ibicuruzwa byiza mu murima wacu kugirango duhure nabakiriya bakeneye cyane kubushakashatsi bukunzwe kubushinwa 96t Automatic DNA RnaAcide Nucleic AcideIgisubizo hamwe na CE na FDA, Twishimiye ibyiringiro byo gukora ubucuruzi buciriritse hamwe nawe kandi twizera ko tuzishimira guhuza nibindi bicuruzwa byacu.
Igishushanyo Cyamamare KuriUbushinwa Nucleic Acide Isolation Solution, Nucleic Acide Isolation, Kubantu bose bashishikajwe nibintu ibyo aribyo byose ukimara kubona urutonde rwibicuruzwa byacu, ugomba rwose kumva ufite umudendezo rwose kugirango utumenyeshe ibibazo.Urashobora kutwoherereza imeri hanyuma ukatwandikira kugirango tugishe inama kandi tuzagusubiza vuba bishoboka.Niba byoroshye, urashobora kumenya aderesi yacu kurubuga rwacu hanyuma ukaza mubucuruzi bwacu kubindi bisobanuro byinshi byibicuruzwa byacu wenyine.Twama twiteguye kubaka umubano mugari kandi uhamye wubufatanye nabakiriya bose bashoboka mubice bifitanye isano.