Ubushinwa OEM / ODM Uruganda Ubushinwa Magnetic Isaro Uburyo bwa Nucleic Acide Rna Gukuramo Reagent Ibikoresho Kubihe Byukuri PCR Kumenyekanisha no gutanga ibicuruzwa |Foregene

Uruganda rwa OEM / ODM Ubushinwa Ububiko bwa Magnetic Amashanyarazi Uburyo bwa Nucleic Acide Rna Gukuramo Reagent Ibikoresho Kubihe Byukuri PCR Kumenya

Uruganda rwa OEM / ODM Ubushinwa Magnetic Amasaro Uburyo Nucleic Acide Rna Gukuramo Reagent Ibikoresho Kubihe Byukuri PCR Kumenyekanisha Ishusho

Ibisobanuro:

Iki gikoresho gikoresha sisitemu idasanzwe ya lysis buffer kugirango irekure vuba ADN ya genomic kuva umurizo wimbeba, ugutwi, imitsi hamwe nizindi ngero za tissue kubitekerezo bya PCR, kubwibyo birakwiriye cyane cyane kwipimisha rinini.

Igikorwa cyo kurekura ADN genomic muri buffer ya lysis kirangiye muminota 10-30 kuri 65 ° C.Ntayindi nzira nka protein na RNA ikuraho, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.

2 × M1-PCR EasyTM ivanze (UNG) ikoresha dUTP aho gukoresha dTTP hashingiwe kuri 2 × M1-PCR EasyTM Mix (UNG), ikongeramo enzyme ya UNG (Uracil-N-glycosylase) ishobora gutesha agaciro inyandikorugero irimo dUTP icyarimwe.Mbere ya PCR reaction, enzyme ya UNG ikoreshwa mugutesha agaciro ibicuruzwa bya PCR birimo uracil.Enzyme ya UNG ntacyo izagira ku gishushanyo kitarimo uracil, bityo kikaba cyemeza umwihariko n'ukuri kwa amplification no gukumira ko hashobora kubaho ibizamini binini.Kwanduza ibicuruzwa bya PCR byabaye.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ubucuruzi bwacu bushingiye ku ihame shingiro rya "Ubwiza bushobora kuba ubuzima hamwe n’ikigo, kandi ibyanditswe bizakubera ubugingo bwabyo" ku ruganda rwa OEM / ODM Ubushinwa Magnetic Beads Method Nucleic Acide Rna Gukuramo Reagent Kits for Real Time PCR Kumenya, Abakozi bacu bafite intego yo gutanga ibisubizo hamwe nigiciro kinini cyibikorwa byacu mubyifuzo byacu, ndetse no kubatugezaho isi yose.
Ubucuruzi bwacu bushingiye ku ihame shingiro rya "Ubwiza bushobora kuba ubuzima hamwe na firime, kandi ibyanditswe bizaba ubugingo bwabyo" kuriAutomatic Nucleic Acide, Ubushinwa Universal Rna ADN Reagents, Muri iki gihe ibicuruzwa byacu bigurishwa hirya no hino mu gihugu ndetse no hanze yacyo tubikesha ubufasha busanzwe kandi bushya kubakiriya.Turerekana ibicuruzwa byiza kandi nibiciro byapiganwa, twakire abakiriya basanzwe kandi bashya bafatanya natwe!

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.	Depite Buffer
	Foregene Protease Yongeyeho
	6 × ADN Yipakurura Buffer
Igice cya II	2 × M1 PCR Biroroshye^TMKuvanga (UNG)
Amabwiriza

Ibiranga ibyiza

■ Nta bisabwa bitwara igihe kandi bihenze bya ADN bisabwa.

Icyitegererezo gikenewe ni gito, nkibice 5mg byimbeba cyangwa umurizo wimbeba 2-5mm.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

System Sisitemu yo kurwanya umwanda PCR 2 × M1-PCR Byoroshye^TMKuvanga (UNG), bikuraho neza umwanda uterwa nibicuruzwa bya PCR kandi ukemeza umwihariko nukuri kwa amplification.

Ibipimo by'ibikoresho

Igipimo cyo gusaba: umurizo wimbeba, ugutwi kwimbeba, imitsi nizindi ngingo.

ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.

Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.

Urujya n'uruza rw'akazi

wk flw-imbeba umurizo PCR

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.

Buffer AL reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.

Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.

Reagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.

Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.

Reagent 2 × M1 PCR EasyTM Ivanga (UNG), iyo ikoreshejwe kenshi, irashobora kandi kubikwa kuri 4 ℃ kugirango ibike igihe gito (ikoreshwe mu minsi 10) .Ibikorwa byacu byubucuruzi ku ihame shingiro rya "Ubwiza bushobora kuba ubuzima hamwe nikigo, kandi inyandiko zerekana inzira zizaba ubugingo bwacyo" kubakozi ba OEM / ODM Uruganda rukora Rukuruzi Ruto hamwe nigiciro kinini cyibiciro ugereranije nibyifuzo byacu, kimwe nintego kuri twese burigihe ni uguhaza abakiriya bacu aho bari hose kwisi.
Uruganda rwa OEM / ODMUbushinwa Universal Rna ADN Reagents, Automatic Nucleic Acide, Muri iki gihe ibicuruzwa byacu bigurishwa hirya no hino mu gihugu ndetse no hanze yacyo tubikesha ubufasha busanzwe kandi bushya kubakiriya.Turerekana ibicuruzwa byiza kandi nibiciro byapiganwa, twakire abakiriya basanzwe kandi bashya bafatanya natwe!

Mbere: Uruganda rwa ODM Ubushinwa Mtt Kumenya Kit 1000t Cell Viability Detection Reagent

Ibikurikira: Uruganda rwa OEM / ODM Ubushinwa dNTP MIX (10mM)

Andika ubutumwa bwawe hano hanyuma utwohereze