Kurenga kimwe cya kabiri cya 2022, ngwino urebe ingingo zatsindiye amanota menshi zashyizweho na Foeregene!
01
NIBA ： 17.694
Byanditswe na: Ibitaro Byambere Bishamikiyeho, Kaminuza Yat-sen
Ibyingenzi byingenzi: Esophageal squamous selile carcinoma (ESCC) nubwoko nyamukuru bwa kanseri yibasira.Kumenya intego nziza zo kuvura ningirakamaro cyane mukuvura neza ESCC.Ubushakashatsi bumwe bwerekanye ko mismodification ya RNA ishobora guteza imbere imvugo no guhindura mRNA zifitanye isano na kanseri, ariko uburyo bwa molekile ntibusobanutse neza.Ubu bushakashatsi bwerekanye ko tRNA m7G methyltransferase ifitanye isano na poroteyine zikomeye METTL1 na WDR4 ziyongereye cyane mu ngingo za ESCC kandi bigira uruhare mu kumenyekanisha nabi kwa ESCC.Kuva icyo gihe, ubushakashatsi bwa gene knockout ya METTL1 na WDR4 mu mbeba z'icyitegererezo cya ESCC bwemeje ko ibikorwa byongerewe imbaraga bya tRNA m7G methyltransferase biganisha ku kugenzura imikorere ya poroteyine zombi, ari nazo ziteza imbere ikwirakwizwa ry'utugingo ngengabuzima twa ESCC.Ubu bushakashatsi bwerekana imikorere yingenzi ya oncogenic yo guhindura tRNA m7G itemewe muri ESCC kandi irerekana ko kwibasira METTL1 ninzira zayo zerekana inzira zishobora kuba intego zo kuvura ESCC.
Ibicuruzwa bifashwa:
Imbeba umurizo Direct PCR Kit Foregene# TP-01333
Ihuza ry'umwimerere:
https://www.umuco.com/articles/s41467-022-29125-7
02
NIBA ： 14.903
Byanditswe na: Laboratoire ya Leta y’ubushakashatsi bufite ireme mu buvuzi bw’Ubushinwa, Ikigo cy’ubuvuzi cy’Ubushinwa, Kaminuza ya Macau
Ibyingenzi:
Hamwe niterambere ryikoranabuhanga rya sintetike, mRNA yabaye icyiciro gishya cyimiti ivura.Nyamara, kugera ku buryo bunoze bwo gutanga imiti ya mRNA bikomeje kuba ingorabahizi kubera kumva mRNA kuri RNase.Ubu bushakashatsi butanga gahunda nziza yo gutanga ya polifenol-conjugated nanostructures ishobora gutanga neza IL-10 mRNA muri vivo.Nyuma yo gukoresha iyi nanostructure kugirango itange imiti ya mRNA, urwego rwa IL-10 rwagenzuwe byumwihariko, kubuza neza kwerekana ibintu bitera umuriro, guteza imbere gusana ururenda, kurinda ingirabuzimafatizo ya epiteliyale na apoptose, no kubuza dextran sodium sulfate iterwa na acite na chronique ntoya ya murine colitis ifite ingaruka zikomeye zo kuvura indwara zifata amara.
Ibicuruzwa bifashwa:
Amatungo Yuzuye RNA Yigunga Kit Foregene# RE-03011
Ihuza ry'umwimerere:
03
NIBA ： 12.499
Byanditswe na: Laboratoire yubushakashatsi bwindwara zinyamaswa, Ishuri Rikuru ryubuvuzi bwamatungo, kaminuza yubuhinzi ya Sichuan
Ibyingenzi:
Guhangayikishwa n'intanga ngabo ni ikintu gikomeye kiganisha ku kutabyara kw'abagabo, bityo rero ni uburyo bushoboka kandi bwiza bwo gushyiraho imiterere y’inyamanswa z’abagabo zidafite ubugumba uhindura sisitemu ya antioxydeant mu buryo bugamije.Nyamara, uburyo bwa antioxydeant ya mikorobe yumugabo ntabwo bwigeze busobanurwa.Ubu bushakashatsi bwemeje ko gene ya LanCL1 ari gene yihariye ya antioxydants mu ngirabuzimafatizo z’abagabo, kandi imvugo yayo iragabanuka cyangwa ifitanye isano n’ubugumba bw’umugabo.Imbeba zabagabo zabuze gene ya LanCL1 zigaragaza kwangirika kwintanga ngabo no kwangirika kwuburumbuke.Muburyo bwo gutandukanya intanga ngirabuzimafatizo, iyo okiside imaze kugaragara, poroteyine yihariye ya poroteyine 1 (SP1) izagenga imiterere ya gene ya LanCL1, bityo ibuze inzira ya okiside yintanga.Ubu bushakashatsi bwerekana ko inzira ya SP1-LanCL1 igira uruhare runini mugutunganya testosticular homeostasis nuburumbuke bwumugabo byahujwe nuburinganire bwa redox, kandi byerekana ko imbeba zahinduwe na LanCL1 zifite uburyo bukurura nkicyitegererezo cyinyamaswa zuburumbuke bwumugabo.
Ibicuruzwa bifashwa:
Imbeba umurizo ADN Mini Kit Foregene# DE-05211
Ihuza ry'umwimerere:
http://europepmc.org/article/MED/35469022
◮Ntabwo bitwara igihe kandi bihenze bya ADN bisabwa.
SampleIcyitegererezo gisabwa ni gito, fata imbuto imwe.
◮Ntabwo buryo bwihariye bwo kuvura nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.
Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.
Inyamaswa Igiteranyo Cyuzuye RNA
ONtabwo ukeneye guhangayikishwa no kwangirika kwa RNA.Sisitemu yose ni RNase-Yubusa.
Kuraho neza ADN ukoresheje ADN-Isukura.
Kuraho ADN utongeyeho DNase.
Byoroheje - ibikorwa byose birangirira ku bushyuhe bwicyumba.
Kwihuta - ibikorwa birashobora kurangira muminota 30.
Umutekano-nta reagent ikoreshwa.
Isuku ryinshi - OD260 / 280≈1.8-2.1.
◮Nta kwanduza RNase:Inkingi ya ADN Yonyine itangwa nigikoresho ituma bishoboka kuvana RNA muri ADN genomic utongeyeho RNase mugihe cyigeragezo, ikabuza laboratoire kwanduzwa na RNase idasanzwe.
◮Umuvuduko wihuse:Foregene Protease ifite ibikorwa byinshi kurenza protease isa, kandi igogora ingero za tissue vuba;igikorwa kiroroshye, kandi ibikorwa byo gukuramo ADN genomic birashobora kurangira muminota 20-80.
◮Byoroshye:Centrifugation ikorerwa mubushyuhe bwicyumba, kandi ntihakenewe 4 ° C ubushyuhe buke bwa centrifugation cyangwa imvura ya Ethanol ya ADN.
◮Umutekano:Nta gukuramo ibinyabuzima bisabwa.
◮Ubwiza bwo hejuru:ADN yakuweho ADN ifite ibice binini, nta RNA, nta RNase, hamwe na ion nkeya cyane, bishobora kuzuza ibisabwa mubushakashatsi butandukanye.
◮Sisitemu yo gukuraho Micro:Irashobora kongera ubunini bwa ADN ya genomic, ikaba yoroshye yo gutahura cyangwa kugerageza.
Igihe cyo kohereza: Nzeri-13-2022
