Ivumburwa rya moteri ya parike yazanye impinduramatwara mu nganda, iterambere rya mudasobwa ryaremye isi ya none, kandi impinduramatwara yikoranabuhanga izazana iterambere ryibihe!Ivuka ryaAkagari ka RT-qPCR Kitya Foregene iri hafi kuzana ibihe bishya byo gutahura molekile!

7min Kubona icyitegererezo cya RNA

Nibyo, wabyumvise neza, ntugashidikanya, kuva selile kugeza RT-qPCR, ubu intambwe ebyiri gusa

Hagarika reaction

Q:Gukuramo RNA birahagije kumuvuduko wihuse?Ese hazabaho kwanduza ADN?

A: Ntugire impungenge, ibicuruzwa byacu byagereranijwe no kugenzura bitabarika, kandi nta tandukaniro ugereranije nibisubizo byubushakashatsi bwa RT-qPCR.Byongeye kandi, ingano yicyitegererezo ni nto, kandi igeragezwa rirashobora gukorwa byibuze selile 10 zifite imico.Yaba isahani ya 6, 12, 24 cyangwa 96-iriba, selile zirashobora guterwa neza mumariba yisahani, byoroshye kandi byihuse.Hamwe na 5 × Direct RT ivanze na 2 × Direct qPCR ivanze mubicuruzwa, inyandikorugero ya RNA irashobora gukoreshwa muburyo bwo guhindura ibintu neza no kwongerera imbaraga, hamwe na sensibilité yo hejuru, umwihariko ukomeye hamwe no guhagarara neza.Ibisobanuro byanditse bya qPCR biroroshye cyane.

Akagari ka RT-qPCR Kitntabwo ifite sisitemu yo hejuru ya lysis gusa, ariko kandi ifite imbaraga zikomeye zo kurwanya RT-qPCR inhibitor reaction, ituma ibikoresho byose bifite imipaka mike yo gutahura, kandi irashobora kubona ibisubizo byiza kuri selile 10.Iyi ntambwe imwe RT qPCR kit iroroshye cyane.

Noneho hariho SYBR icyatsi kibisi qPCR na Taqman qPCR uburyo burahari.