Bikaba bifite imyifatire myiza kandi igenda itera imbere gushimisha abakiriya, uruganda rwacu ruhora rutezimbere ibintu byacu byiza kugirango duhaze ibyifuzo byabakiriya kandi tunibanda cyane kumutekano, kwiringirwa, ibisabwa kubidukikije, no guhanga udushya twakozwe nu Bushinwa Magnetic Beads Nucleic Acide PCR Ikuramo ibikoresho hamwe na CE kumashini ya Poct PCR, Turizera rwose ko dukura hamwe nibyifuzo byacu mubidukikije.
Bikaba bifite imyumvire myiza kandi igenda itera imbere gushimisha abakiriya, uruganda rwacu ruhora rutezimbere ibintu byacu byiza kugirango duhaze ibyifuzo byabakiriya kandi bikomeza kwibanda kumutekano, kwiringirwa, kubidukikije, no guhanga udushya.Ubushinwa bwo gukuramo ADN, Gukuramo Acide Nucleic, Twubahiriza umukiriya wa 1, ubuziranenge bwo hejuru 1, guhora utezimbere, inyungu zinyuranye hamwe no gutsindira inyungu.Iyo ubufatanye hamwe nabakiriya, dutanga abaguzi hamwe na serivise nziza yo hejuru.Gushiraho umubano mwiza wubucuruzi ukoresheje umuguzi wa Zimbabwe imbere mubucuruzi, twabonye ikirango n'icyubahiro.Mugihe kimwe, mwakire tubikuye ku mutima ibyifuzo bishya kandi bishaje muri sosiyete yacu yo kujya no kuganira kubucuruzi buciriritse.

Ibisobanuro

Iki gikoresho gikoresha sisitemu idasanzwe ya lysis buffer ishobora kurekura byihuse RNA mumikorere yimikorere yimikorere ya RT-qPCR, bityo bikuraho inzira yo kweza RNA itwara igihe kandi ikora cyane.Inyandikorugero ya RNA irashobora kuboneka muminota 7 gusa.5 × Direct RT ivanze na 2 × Imiyoboro ya qPCR ivanze-SYBR itangwa nigitabo irashobora kwihuta kandi neza kubona ibisubizo nyabyo-byuzuye bya PCR.

5 × Direct RT ivanze na 2 × Direct qPCR ivanze-SYBR ifite kwihanganira imbaraga za inhibitor, kandi lysate yintangarugero irashobora gukoreshwa nkicyitegererezo cya RT-qPCR itaziguye.Iki gikoresho kirimo RNA idasanzwe-yuzuye ya Foregene reverse transcriptase, hamwe na polymerase ya D-Taq ishyushye, DNTPs, MgCl₂, reaction ya reaction, optimizer ya PCR na stabilisateur.

Ibisobanuro

200 × 20μl Rxns, 1000 × 20μl Rxns

Ibigize ibikoresho

Ibiranga ibyiza

■ Byoroshye kandi byiza: hamwe na tekinoroji ya Direct Direct RT, ingero za RNA zirashobora kuboneka muminota 7 gusa.

Icyitegererezo gikenewe ni gito, nkuko selile 10 zishobora kugeragezwa.

Thr Kwinjiza cyane: irashobora kumenya vuba RNA mu ngirabuzimafatizo zifite 384, 96, 24, 12, 6-amasahani meza.

Er ADN Eraser irashobora gukuraho vuba genome zasohotse, bikagabanya cyane ingaruka kubisubizo byakurikiyeho.

System Optimized RT na qPCR sisitemu ituma intambwe ebyiri RT-PCR ihinduranya transcript neza kandi PCR ikarushaho kuba nziza, kandi ikarwanya RT-qPCR inhibitor.

Porogaramu

Igipimo cyo gushyira mu bikorwa: ingirabuzimafatizo.

- RNA yarekuwe na sample lysis: ikoreshwa gusa kuri RT-qPCR inyandikorugero yiki gitabo.

- Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: gusesengura imvugo ya gene, kugenzura ingaruka zo gucecekesha gene ya siRNA, gusuzuma ibiyobyabwenge, nibindi.

Igishushanyo

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 4 ℃;Igice cya II kigomba kubikwa kuri -20 ℃.

Foregene Protease Plus II igomba kubikwa kuri 4 ℃, ntugahagarike kuri -20 ℃.

Reagent 2 × Direct qPCR Ivanga-SYBR igomba kubikwa kuri -20 ℃ mu mwijima;iyo ikoreshejwe kenshi, irashobora kandi kubikwa kuri 4 ℃ kugirango ibike igihe gito (koresha mugihe cyiminsi 10) .Ni ikihe gifite imyumvire myiza kandi igenda itera imbere mugushimisha abakiriya, uruganda rwacu ruhora rutezimbere ibintu byiza cyane kugirango duhaze ibyifuzo byabakiriya kandi rwibanda cyane kumutekano, kwiringirwa, ibisabwa mubidukikije, no guhanga udushya twakozwe na Chine Magnetic Beads Nucleic Acide PCR Gukuramo ibikoresho hamwe na CE kuri Poct PCR Machine.
Uruganda rwaUbushinwa bwo gukuramo ADN, Gukuramo Acide Nucleic, Twubahiriza umukiriya wa 1, ubuziranenge bwo hejuru 1, guhora utezimbere, inyungu zinyuranye hamwe no gutsindira inyungu.Iyo ubufatanye hamwe nabakiriya, dutanga abaguzi hamwe na serivise nziza yo hejuru.Gushiraho umubano mwiza wubucuruzi ukoresheje umuguzi wa Zimbabwe imbere mubucuruzi, twabonye ikirango n'icyubahiro.Mugihe kimwe, mwakire tubikuye ku mutima ibyifuzo bishya kandi bishaje muri sosiyete yacu yo kujya no kuganira kubucuruzi buciriritse.