Mubisanzwe dukora kuba abakozi bakomeye kugirango tumenye neza ko tuzaguha ibyiza byingirakamaro hiyongereyeho igiciro cyiza cyo kugurisha Igicuruzwa gishyushye kubushinwa PCR Nucleic Acide Test Kit hamwe na Fluorescent Rt PCR Ikizamini cya Laboratoire, Murakaza neza abo mwashakanye baturutse impande zose z'isi bibaho kujya, kubitabo no kuganira.
Mubisanzwe dukora kuba abakozi bakora neza kugirango tumenye neza ko tuzaguha ibyiza cyane wongeyeho igiciro cyiza cyo kugurishaIgipimo cyo Gusuzuma Ubushinwa, Ikizamini cyo Gusuzuma Byihuse, Dufite intego yo "guhatanira ubuziranenge bwiza no kwiteza imbere no guhanga udushya" hamwe nihame rya serivisi yo "gufata ibyifuzo byabakiriya nkicyerekezo", tugiye gutanga byimazeyo ibisubizo byujuje ubuziranenge na serivisi nziza kubakiriya bo mu gihugu ndetse n’amahanga.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Virus RNA Nucleic Acide Gukuramo Gukuramo Isolation Kit ikoresha inkingi ya spin hamwe na formula yakozwe na Foregene, ishobora gukuramo neza virusi ya RNA ifite isuku nziza kandi yujuje ubuziranenge mu ngero nka plasma, serumu, amazi adafite selile, hamwe n’umuco ndengakamere.Igikoresho kongeramo byumwihariko Linear Acrylamide, ishobora gufata byoroshye RNA nkeya mubitegererezo.RNA-Inkingi yonyine irashobora guhuza RNA neza.Igikoresho gishobora gutunganya umubare munini wicyitegererezo icyarimwe.

Ibikoresho byose ntabwo birimo RNase, bityo RNA yatunganijwe ntizitesha agaciro.Buffer viRW1 na Buffer viRW2 birashobora kwemeza ko aside nucleic yabonetse yabonetse idafite proteyine, nuclease cyangwa iyindi myanda, ishobora gukoreshwa mu buryo butaziguye mu bushakashatsi bw’ibinyabuzima bwa molekile.

Ibigize ibikoresho

Ibiranga ibyiza

Gukorera mubushyuhe bwicyumba (15-25 ℃) mubikorwa byose, nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Umusaruro mwinshi wa aside nucleique: ADN / RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza ADN na RNA.
Ubushobozi bunini bwo gutunganya: urugero rwa 200μl rushobora gutunganywa buri gihe.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 20.
Umutekano: nta reagent ngenga isabwa.
Quality Ubwiza buhanitse: Ibice bya RNA bisukuye bifite isuku nyinshi, bitarimo poroteyine n’ibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.

Porogaramu

Irakwiriye gukuramo no kweza aside nucleic virusi mu ngero nka plasma, serumu, umubiri utagira selile hamwe numuco ndengakamere.

Urujya n'uruza rw'akazi

Igishushanyo

Ububiko hamwe nubuzima bwa Shelf

■ Iki gikoresho gishobora kubikwa amezi 24 mugihe cyumye mubushyuhe bwicyumba (15-25 ℃);niba ikeneye kubikwa igihe kirekire, irashobora kubikwa muri 2-8 ℃.
Solution Umurongo wa Acrylamide urashobora kubikwa mubushyuhe bwicyumba muminsi 7;nyuma yo kwakira ibikoresho, nyamuneka kuyikuramo no kubika kuri -20 ° C.
■ Nyuma yo kongeramo Linear Acrylamide muri Buffer DRL, irashobora kubikwa kuri 2-8 ° C kugeza kuri 48h.Nyamuneka koresha igisubizo cyiteguye.

Mubisanzwe dukora kuba abakozi bakomeye kugirango tumenye neza ko tuzaguha ibyiza byingirakamaro hiyongereyeho igiciro cyiza cyo kugurisha Igicuruzwa gishyushye kubushinwa PCR Nucleic Acide Test Kit hamwe na Fluorescent Rt PCR Ikizamini cya Laboratoire, Murakaza neza abo mwashakanye baturutse impande zose z'isi bibaho kujya, kubitabo no kuganira.
Igurisha rishyushye kuriIgipimo cyo Gusuzuma Ubushinwa, Ikizamini cyo Gusuzuma Byihuse, Dufite intego yo "guhatanira ubuziranenge bwiza no kwiteza imbere no guhanga udushya" hamwe nihame rya serivisi yo "gufata ibyifuzo byabakiriya nkicyerekezo", tugiye gutanga byimazeyo ibisubizo byujuje ubuziranenge na serivisi nziza kubakiriya bo mu gihugu ndetse n’amahanga.