Ibyo bifite inguzanyo ntoya yubucuruzi, serivise nziza nyuma yo kugurisha hamwe nibikorwa bigezweho byo gukora, twabonye umwanya wingenzi hagati yabaguzi bacu kwisi yose kugirango Ubushobozi Bwiza Bwubushinwa Bworoheye Tissue Genomic ADN Nucleic Acide Extraction Isukura Reagents, Mugihe urimo gushakisha ubuziranenge, bwihuse, byiza nyuma yubufasha hamwe nogutanga agaciro keza mubushinwa kugirango uhuze ibikorwa byigihe kirekire, tugiye guhitamo neza.
Ibyo bifite inguzanyo ntoya yubucuruzi, serivisi nziza nyuma yo kugurisha hamwe nibikorwa bigezweho, twatsindiye umwanya mwiza hagati yabaguzi bacu kwisi yoseAutomatic Nucleic Acide Isukura, Ubushinwa Tissue Genomic ADN Kit, Ibicuruzwa byacu byatsindiye izina ryiza muri buri gihugu gifitanye isano.Kuberako ishyirwaho ryikigo cyacu.twatsimbaraye kubikorwa byacu byo guhanga udushya hamwe nuburyo bugezweho bwo gucunga uburyo bugezweho, dukurura impano zitari nke muri uru ruganda.Dufata igisubizo cyiza nkimiterere yacu yingenzi.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Ibiranga ibyiza

■ Nta bisabwa bitwara igihe kandi bihenze bya ADN bisabwa.

Icyitegererezo gikenewe ni gito, nkibice 5mg byimbeba cyangwa umurizo wimbeba 2-5mm.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

System Sisitemu yo kurwanya umwanda PCR 2 × M1-PCR Byoroshye^TMKuvanga (UNG), bikuraho neza umwanda uterwa nibicuruzwa bya PCR kandi ukemeza umwihariko nukuri kwa amplification.

Ibipimo by'ibikoresho

Igipimo cyo gusaba: umurizo wimbeba, ugutwi kwimbeba, imitsi nizindi ngingo.

ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.

Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.

Urujya n'uruza rw'akazi

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.

Buffer AL reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.

Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.

Reagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.

Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.

Reagent 2 × M1 PCR EasyTM ivanze (UNG), iyo ikoreshejwe kenshi, irashobora kandi kubikwa kuri 4 ℃ kugirango ibike igihe gito (koresha muminsi 10) .Ibyo bifite inguzanyo ntoya yubucuruzi, serivise nziza nyuma yo kugurisha hamwe nibikorwa bigezweho, twabonye ibihembo bihagaze neza hagati yabaguzi bacu kwisi yose kugirango ubashe kubona ibintu byiza cyane Kubona ibintu Byiza cyane, utanga agaciro mubushinwa kugirango uhuze igihe kirekire mumashyirahamwe, tugiye guhitamo neza.
Imikorere yo hejuruUbushinwa Tissue Genomic ADN Kit, Automatic Nucleic Acide Isukura, Ibicuruzwa byacu byatsindiye izina ryiza muri buri gihugu gifitanye isano.Kuberako ishyirwaho ryikigo cyacu.twatsimbaraye kubikorwa byacu byo guhanga udushya hamwe nuburyo bugezweho bwo gucunga uburyo bugezweho, dukurura impano zitari nke muri uru ruganda.Dufata igisubizo cyiza nkimiterere yacu yingenzi.