Turashoboye guhaza muri rusange abakiriya bacu bubashywe nibyiza byacu byiza, igiciro cyiza kandi ninkunga nziza cyane kuberako twabaye inzobere cyane kandi twongerewe imbaraga kandi turabikora muburyo buhendutse bwo kugurisha uruganda rwo mu Bushinwa Plastike Magnetic Bead Method Universal Nucleic Acide Extraction Rapid Kit, Ibicuruzwa byacu birasuzumwa cyane mbere yo kohereza hanze, bityo tukabona izina ryiza kwisi yose.Dutegereje ubufatanye nawe ejo hazaza.
Turashoboye guhaza muri rusange abakiriya bacu bubashywe nubwiza bwacu bwiza cyane, igiciro cyiza kandi ninkunga nziza cyane kuberako twabaye inzobere cyane kandi dukora cyane kandi tubikora muburyo buhendutse kuriUbushinwa Gukuramo Acide Nucleic Universal, Gukuramo Acide ya Nucleic Acide, Isosiyete yacu yubahiriza igitekerezo cyo kuyobora "komeza udushya, ukurikirane indashyikirwa".Dushingiye ku kwemeza ibyiza byibisubizo bihari, dukomeza gushimangira no kwagura iterambere ryibicuruzwa.Isosiyete yacu ishimangira udushya kugira ngo duteze imbere iterambere rirambye ry’imishinga, kandi itume duhinduka abatanga isoko ryiza mu gihugu.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Igikoresho gikoresha spin inkingi hamwe na formula yakozwe na Foregene, ishobora gukuramo neza-isuku nziza kandi yujuje ubuziranenge RNA yose mubice bitandukanye byibimera bifite polysaccharide nkeya hamwe na polifenol.Kubitegererezo byibihingwa bifite polysaccharide nyinshi cyangwa polifenole, birasabwa gukoresha Plant Total RNA Isolation Plus Kit kugirango ubone ibisubizo byiza byo gukuramo RNA.Igikoresho gitanga ADN-Isukura inkingi ishobora gukuraho ADN genomic muri lysate ndengakamere na tissue.Inkingi ya RNA yonyine irashobora guhuza RNA neza.Igikoresho gishobora gutunganya umubare munini wicyitegererezo icyarimwe.

Sisitemu yose ntabwo irimo RNase, bityo RNA yatunganijwe ntizitesha agaciro.Buffer PRW1 na Buffer PRW2 birashobora kwemeza ko RNA yabonye itanduye na poroteyine, ADN, ion, hamwe n’ibinyabuzima.

Ibigize ibikoresho

Ibiranga ibyiza

Gukorera mubushyuhe bwicyumba (15-25 ℃) mubikorwa byose, nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byuzuye RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura Inkingi ihuza cyane na ADN, kugirango ibikoresho bishobore gukuraho ADN yanduye utongeyeho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 30.
Umutekano: nta reagent ngenga isabwa.
Quality Ubwiza buhanitse: Ibice bya RNA bisukuye bifite isuku nyinshi, bitarimo poroteyine n’ibindi byanduye, kandi birashobora guhura nuburyo butandukanye bwo kugerageza.

Porogaramu

Birakwiriye gukuramo no kweza RNA yose uhereye kumyumbati y'ibiti bishya cyangwa bikonje (cyane cyane ibibabi byibabi bishya) bifite polysaccharide nkeya hamwe na polifenol.

Urujya n'uruza rw'akazi

Igishushanyo

Gutera Igiteranyo cya RNA Isolation Kit Plus yatunganije 50mg yamababi mashya ya polysaccharide na polifenol, naho 5% RNA yatunganijwe yapimwe na electrophorei.
1: Umuneke
2: Ginkgo
3: Impamba
4: Amakomamanga

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) ahantu humye, na 2-8 ℃ mugihe kirekire (amezi 24).

Buffer PSL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka).

Turashoboye guhaza muri rusange abakiriya bacu bubashywe nibyiza byacu byiza, igiciro cyiza kandi ninkunga nziza cyane kuberako twabaye inzobere cyane kandi twongerewe imbaraga kandi turabikora muburyo buhendutse bwo kugurisha uruganda rwo mu Bushinwa Plastike Magnetic Bead Method Universal Nucleic Acide Extraction Rapid Kit, Ibicuruzwa byacu birasuzumwa cyane mbere yo kohereza hanze, bityo tukabona izina ryiza kwisi yose.Dutegereje ubufatanye nawe ejo hazaza.
Uruganda rwinshiUbushinwa Gukuramo Acide Nucleic Universal, Gukuramo Acide ya Nucleic Acide, Isosiyete yacu yubahiriza igitekerezo cyo kuyobora "komeza udushya, ukurikirane indashyikirwa".Dushingiye ku kwemeza ibyiza byibisubizo bihari, dukomeza gushimangira no kwagura iterambere ryibicuruzwa.Isosiyete yacu ishimangira udushya kugira ngo duteze imbere iterambere rirambye ry’imishinga, kandi itume duhinduka abatanga isoko ryiza mu gihugu.