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Uruganda rutaziguye Ubushinwa Nucleic Acide Byihuta PCR Ikizamini cyo Kugenzura Virusi Murugo

Uruganda rutaziguye Ubushinwa Nucleic Acide Byihuta PCR Ikizamini cyo Kugenzura Virusi Murugo Murugo Ishusho
  • Uruganda rutaziguye Ubushinwa Nucleic Acide Byihuta PCR Ikizamini cyo Kugenzura Virusi Murugo
  • Uruganda rutaziguye Ubushinwa Nucleic Acide Byihuta PCR Ikizamini cyo Kugenzura Virusi Murugo
  • Uruganda rutaziguye Ubushinwa Nucleic Acide Byihuta PCR Ikizamini cyo Kugenzura Virusi Murugo
  • Uruganda rutaziguye Ubushinwa Nucleic Acide Byihuta PCR Ikizamini cyo Kugenzura Virusi Murugo

Ibisobanuro:

Iki gikoresho gikoresha sisitemu idasanzwe ya lysis buffer kugirango irekure vuba ADN ya genomic kuva umurizo wimbeba, ugutwi, imitsi hamwe nizindi ngero za tissue kubitekerezo bya PCR, kubwibyo birakwiriye cyane cyane kwipimisha rinini.

Igikorwa cyo kurekura ADN genomic muri buffer ya lysis kirangiye muminota 10-30 kuri 65 ° C.Ntayindi nzira nka protein na RNA ikuraho, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.

2 × M1-PCR EasyTM Ivanga ifite kwihanganira bikomeye PCR ibuza reaction, kandi irashobora gukoresha lysate yicyitegererezo kugirango igeragezwe nkicyitegererezo cyo gukora neza kandi cyihariye.Iyi reagent irimo Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, buffer reaction, optimizer ya PCR na stabilisateur.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.


Ibicuruzwa birambuye

Ibicuruzwa

Dutsimbaraye kuri "Ubwiza buhebuje, Gutanga Byihuse, Igiciro Cy’ibitero", ubu twashyizeho ubufatanye burambye hamwe n’abaguzi baturutse mu mahanga kimwe ndetse no mu gihugu imbere kandi tubona ibitekerezo bishya by’abakiriya bashya ku ruganda rutaziguye Ubushinwa Nucleic Acide Rapid PCR IkizaminiKugenzura virusiIgikoresho murugo, Ikaze ikibazo icyo aricyo cyose mubigo byacu.Tugiye kwishimira gushiraho imikoranire myiza yubucuruzi nawe!
Dutsimbaraye kuri "Ubwiza buhebuje, Gutanga Byihuse, Igiciro cyo Kwibabaza", ubu twashizeho ubufatanye burambye hamwe n’abaguzi baturutse mu mahanga kimwe no mu gihugu imbere kandi tubona ibitekerezo binini kandi bishaje byabakiriya kuriIkizamini cya PCR, Kugenzura virusi, “Shiraho indangagaciro, ukorera abakiriya!”niyo ntego dukurikirana.Turizera rwose ko abakiriya bose bazashiraho ubufatanye burambye kandi bwunguka natwe.Niba wifuza kubona ibisobanuro birambuye kubyerekeye isosiyete yacu, Nyamuneka twandikire nonaha!

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.

Depite Buffer

Foregene Protease Yongeyeho

6 × ADN Yipakurura Buffer

Igice cya II

2 × M1 PCR BiroroshyeTMKuvanga

Amabwiriza

Ibiranga ibyiza

■ Nta bisabwa bitwara igihe kandi bihenze bya ADN bisabwa.

Icyitegererezo gikenewe ni gito, nkibice 5mg byimbeba cyangwa umurizo wimbeba 2-5mm.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ibipimo by'ibikoresho

Gushyira mu bikorwa: Imbeba umurizo, amatwi yimbeba, imitsi nizindi ngingo.

ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.

Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.

Urujya n'uruza rw'akazi

wk flw-imbeba umurizo PCR

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.

Buffer AL reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.

Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.

Reagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.

Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.

Reagent 2 × M1 PCR ByoroshyeTMKuvanga, niba bikoreshwa kenshi, birashobora kandi kubikwa kuri 4 ℃ kubikwa igihe gito (koresha muminsi 10).

Dutsimbaraye kuri "Ubwiza buhebuje, Gutanga Byihuse, Igiciro Cy’ibitero", ubu twashyizeho ubufatanye burambye hamwe n’abaguzi baturutse mu mahanga kimwe ndetse no mu gihugu imbere kandi tubona ibitekerezo bishya by’abakiriya bashya ku ruganda rutaziguye Ubushinwa Nucleic Acide Rapid PCR IkizaminiKugenzura virusiIgikoresho murugo, Ikaze ikibazo icyo aricyo cyose mubigo byacu.Tugiye kwishimira gushiraho imikoranire myiza yubucuruzi nawe!
Uruganda rutaziguyeIkizamini cya PCRKugenzura virusi, “Kurema Indangagaciro, Gukorera Umukiriya!”niyo ntego dukurikirana.Turizera rwose ko abakiriya bose bazashiraho ubufatanye burambye kandi bwunguka natwe.Niba wifuza kubona ibisobanuro birambuye kubyerekeye isosiyete yacu, Nyamuneka twandikire nonaha!

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