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Uruganda rutaziguye Ubushinwa ISO Yemejwe na laboratoire ya chimie

Uruganda rutaziguye Ubushinwa ISO Yemejwe na laboratoire ya chimie reagents Ishusho Yerekanwe
  • Uruganda rutaziguye Ubushinwa ISO Yemejwe na laboratoire ya chimie
  • Uruganda rutaziguye Ubushinwa ISO Yemejwe na laboratoire ya chimie
  • Uruganda rutaziguye Ubushinwa ISO Yemejwe na laboratoire ya chimie
  • Uruganda rutaziguye Ubushinwa ISO Yemejwe na laboratoire ya chimie

Ibisobanuro:

Iki gikoresho gikoresha sisitemu yihariye ya lysis kugirango irekure vuba ADN genomic muri zebrafish nizindi nyama zamafi yamazi meza, imirizo yumurizo cyangwa amagi y amafi byerekana PCR, bityo rero birakwiriye cyane cyane kwipimisha geneti nini.Igikorwa cyo kurekura ADN genomic muri buffer ya lysis kirangiye muminota 10-30 kuri 65 ° C.Ntayindi nzira nka protein na RNA ikuraho, kandi ADN yarekuwe irashobora gukoreshwa nkicyitegererezo cya PCR.

2 × PCR EasyTM Ivanga ifite kwihanganira bikomeye PCR ibuza gukora, kandi irashobora gukoresha lysate yicyitegererezo kugirango igeragezwe nkicyitegererezo cyo gukora neza kandi cyihariye.Iyi reagent irimo ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, buffer reaction, optimizer ya PCR na stabilisateur.Ikoreshwa ifatanije na lysis buffer irashobora kubona byihuse kandi byoroshye ingero, kandi ifite ibiranga sensibilité yo hejuru, umwihariko ukomeye, hamwe no guhagarara neza.

D-Taq ADN polymerase ni ADN polymerase yakozwe na Foregene kugirango reaction ya PCR itaziguye.D-Taq ADN polymerase yihanganira cyane ibintu bitandukanye byangiza PCR, kandi irashobora kongera imbaraga za ADN muri sisitemu zitandukanye zitoroshye, kandi umuvuduko wa amplification ushobora kugera kuri 2Kb / min, cyane cyane muburyo bwa PCR.


Ibicuruzwa birambuye

Ibicuruzwa

Buri gihe tubona akazi ko kuba abakozi bafatika kugirango tumenye neza ko dushobora kuguha byoroshye ibyiza-byiza kandi byiza cyane ku ruganda Ubushinwa ISO Byemejwelaboratoire ya chimie, Kubindi bibazo cyangwa ufite ikibazo kijyanye nibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira.
Buri gihe tubona akazi ko kuba abakozi bifatika kugirango tumenye neza ko dushobora kuguha byoroshye ibyiza-byiza kandi byiza cyane kurilaboratoire ya chimie, Twateje imbere tekinoroji yumusaruro, kandi dukurikirana udushya mubicuruzwa.Mugihe kimwe, serivisi nziza yazamuye izina ryiza.Twizera ko mugihe usobanukiwe nibicuruzwa byacu, ugomba kuba witeguye kuba abafatanyabikorwa natwe.Dutegereje ibibazo byawe.

Ibisobanuro

50 × 20µl rxns, 200 × 20µl rxns, 500 × 20µl rxns, 2000 × 20µl rxns

Ibigize ibikoresho

Igice cya I.

Buffer FP

Foregene Protease

6 × ADN Yipakurura Buffer

Igice cya II

2 × PCR BiroroshyeTMKuvanga

Amabwiriza

Ibiranga ibyiza

■ Nta bisabwa bitwara igihe kandi bihenze bya ADN bisabwa.

Require Icyitegererezo gikenewe ni gito, nkibishobora 1mg amafi ya caudal fin cyangwa amagi 10 y amafi arashobora gupimwa.

■ Nta buvuzi budasanzwe nko gusya no kumenagura bisabwa, kandi imikorere iroroshye.

System Sisitemu nziza ya PCR ituma PCR igira umwihariko wo kwihanganira no kwihanganira imbaraga za PCR.

Ibipimo by'ibikoresho

Gushyira mu bikorwa: Amafi ya Zebra nandi mafi yamazi meza.

ADN yasohotse muri sample lysis: ikoreshwa gusa nkicyitegererezo cya PCR.

Igikoresho kirashobora gukoreshwa mubikorwa bikurikira: kumenya transgenose, genotyping yinyamaswa, nibindi.

Urujya n'uruza rw'akazi

wk-flw-zebra-ifi-itaziguye-PCR

Ububiko hamwe nubuzima bwa Shelf

Igice cya I cy'iki gikoresho kigomba kubikwa kuri 2-8 ℃.

Buffer FP reagent irashobora kubikwa amezi 12 mugihe cyumye;irashobora kubikwa kuri 2-8 ℃ kugirango ibike igihe kirekire.

v Reagent Foregene Protease ifite formula idasanzwe.Kugirango umenye ibikorwa byayo kandi bihamye, nyamuneka ubike kuri 4 ℃ mumezi 12.

vReagent 6 × ADN Yipakurura Buffer irashobora kubikwa kuri 4 ℃ cyangwa -20 ℃ igihe kirekire.

Igice cya II cyiki gikoresho kigomba kubikwa kuri -20 ℃.

vReagent 2 × PCR EasyTM ivanze, niba ikoreshwa kenshi, irashobora kandi kubikwa kuri 4 ℃ kubikwa igihe gito (koresha muminsi 10).

Buri gihe tubona akazi ko kuba abakozi bafatika kugirango tumenye neza ko dushobora kuguha byoroshye ubuziranenge bwiza kandi bufite agaciro gakomeye ku ruganda mu buryo butaziguye Ubushinwa ISO Yemeje 120tests / H Automatic Electrolyte Analyser na Reagent Price Mslea07, Kubindi bibazo cyangwa niba ufite ikibazo kijyanye nibicuruzwa byacu, nyamuneka ntutindiganye kutwandikira.
Uruganda mu buryo butaziguye Ubushinwa Isesengura rya Electrolyte, Isesengura rya Gaz Amaraso, Twateje imbere ikoranabuhanga ribyara umusaruro, kandi dukurikirana udushya mu bicuruzwa.Mugihe kimwe, serivisi nziza yazamuye izina ryiza.Twizera ko mugihe usobanukiwe nibicuruzwa byacu, ugomba kuba witeguye kuba abafatanyabikorwa natwe.Dutegereje ibibazo byawe.

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