Kugirango tubashe guhaza ibyifuzo byabakiriya, ibikorwa byacu byose bikozwe neza bijyanye nintego yacu "Igiciro cyiza cyo hejuru, Igiciro cyo Kurushanwa, Igiciro cyihuse" cyuruganda ruhendutse Ubushinwa Bwinshi Ibicuruzwa biva mu mahanga Myricetin 529-44-2, Gukomeza kuboneka ibicuruzwa byingenzi byo mu rwego rwo hejuru hamwe n’isoko ryiza cyane mbere na nyuma yo kugurisha bituma isoko ryiyongera cyane ku isoko.
Kugirango tubashe guhaza ibyifuzo byabakiriya, ibikorwa byacu byose birakorwa neza bijyanye nintego yacu "Hejuru-nziza, Igiciro cyo Kurushanwa, Serivise yihuse" kuri529-44-2, Ubushinwa Myricetin, Dufite intego yo "guhatanira ubuziranenge bwiza no guteza imbere guhanga" hamwe nihame rya serivisi yo "gufata ibyifuzo byabakiriya nkicyerekezo", tugiye kwerekana byimazeyo ibicuruzwa byujuje ubuziranenge na serivisi nziza kubakiriya bo mu gihugu ndetse n’amahanga.

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Ibigize ibikoresho

Ibiranga ibyiza

Process Ibikorwa byose bikorerwa mubushyuhe bwicyumba (15-25 ℃), nta bwogero bwa barafu hamwe na centrifugation yubushyuhe buke.
Kit Ibikoresho byose ni RNase-Ubuntu, nta mpamvu yo guhangayikishwa no kwangirika kwa RNA.
Inkingi ya ADN-Isukura ihuza ADN cyane cyane, kugirango igikoresho gishobora gukuraho ADN yanduye utiriwe wongeraho DNase.
Umusaruro mwinshi wa RNA: RNA-Inkingi gusa hamwe na formula idasanzwe irashobora kweza neza RNA.
Speed ​​Umuvuduko wihuse: byoroshye gukora kandi birashobora kurangira muminota 11.
Umutekano: Nta reagent ikenewe.
Quality Ubwiza buhanitse: RNA isukuye ifite isuku nyinshi, idafite poroteyine n’indi mwanda, kandi irashobora guhura nubushakashatsi butandukanye bwakurikiyeho.

Porogaramu

Irakwiriye gukuramo no kweza RNA yose muri selile zifite imico muri plaque 96, 24, 12, na 6.

Urujya n'uruza rw'akazi

Igishushanyo

Igishushanyo cya batiri ya agarose gel ya Cell Total RNA Isolation Kit yavuzeko imibare itandukanye ya selile yavuzwe haruguru, gukuramo 20μl, gufata 2μl isukuye yose hamwe RNA 1%.

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 12 mubushyuhe bwicyumba (15-25 ℃) na 2-8 ℃ mugihe kinini (amezi 24).

Buffer cRL1 irashobora kubikwa kuri 4 ℃ mukwezi 1 nyuma yo kongeramo 2-hydroxy-1-Ethanethiol (bidashoboka). Kugirango tubashe guhaza ibyifuzo byabakiriya, ibikorwa byacu byose birakorwa neza bijyanye nintego yacu "Ibiciro byiza cyane, Ibiciro birushanwe, Serivise yihuse" Ibicuruzwa Byinshi Ubushinwa Bwinshi Bwuzuye Ibicuruzwa Byinshi Ibicuruzwa bikomoka ku bicuruzwa bikomoka ku bicuruzwa 4 inkunga yo kugurisha itanga irushanwa rikomeye ku isoko rigenda ryiyongera ku isi.
Uruganda ruhendutse Ubushinwa Myricetin, 529-44-2, Hagamijwe "guhatanira ubuziranenge bwiza no kwiteza imbere hamwe no guhanga udushya" hamwe nihame rya serivisi yo "gufata ibyifuzo byabakiriya nkicyerekezo", tugiye kwerekana byimazeyo ibicuruzwa byujuje ubuziranenge na serivisi nziza kubakiriya bo mu gihugu ndetse n’amahanga.