Hamwe nibikorwa byizewe, bizwi neza na serivise nziza zabakiriya, urukurikirane rwibicuruzwa byakozwe nisosiyete yacu byoherezwa mubihugu byinshi no mu turere twinshi two mu ruganda ruhendutse Ubushinwa Bwashizwemo na virusi ya ADN Rna yo gukuramo ibikoresho bihuye n’imashini ikuramo 16t 32t 48t 96t, Intego yacu izaba iyo gufasha abaguzi gusobanukirwa intego zabo.Twagiye dukora ibishoboka byose kugirango tubone iyi ntsinzi-kandi turabakuye ku mutima ko mutwiyandikisha.
Hamwe nibikorwa byizewe byizewe, izina ryiza na serivisi nziza zabakiriya, urukurikirane rwibicuruzwa byakozwe nisosiyete yacu byoherezwa mubihugu byinshi no mukarere kuriAutomatic Nucleic Acide, Ubushinwa Universal Rna ADN Reagents, Kugirango wuzuze ibisabwa kubakiriya kugiti cyabo kuri buri biti serivisi nziza nibicuruzwa bihamye.Twishimiye cyane abakiriya kwisi yose kudusura, hamwe nubufatanye bwacu butandukanye, kandi tugafatanya guteza imbere amasoko mashya, tugashiraho ejo hazaza heza!

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo mer- mercaptoethanol (itabishaka) .Nuburyo bwiza bwizewe, izina ryiza hamwe na serivise nziza zabakiriya, urukurikirane rwibicuruzwa byakozwe nisosiyete yacu byoherezwa mubihugu byinshi no mukarere kubucuruzi bwuruganda ruhendutse Ubushinwa Bwuzuye virusi ya Rna Gukuramo ibikoresho Bihuye nintego zabo zo gukuramo 16t 32t 48t 96t.Twagiye dukora ibishoboka byose kugirango tubone iyi ntsinzi-kandi turabakuye ku mutima ko mutwiyandikisha.
Uruganda ruhendutseUbushinwa Universal Rna ADN Reagents, Automatic Nucleic Acide, Kugirango wuzuze ibisabwa kubakiriya kugiti cyabo kuri buri biti serivisi nziza nibicuruzwa bihamye.Twishimiye cyane abakiriya kwisi yose kudusura, hamwe nubufatanye bwacu butandukanye, kandi tugafatanya guteza imbere amasoko mashya, tugashiraho ejo hazaza heza!