Abakozi bacu mubisanzwe bari muburyo bwo "gukomeza gutera imbere no kuba indashyikirwa", kandi mugihe dukoresha ibintu byiza byo mu rwego rwo hejuru bifite ireme ryiza, agaciro keza na serivise nziza nyuma yo kugurisha, turagerageza kubona imyizerere ya buri mukiriya kuri Discount wholesale China Method Rna Nucleic Acide Extraction Kit hamwe nogusukura ibikoresho, Gutanga abakiriya bafite ibikoresho bikomeye nibigo, kandi akenshi biteza imbere imashini nshya nintego zubucuruzi.Dutegereje ubufatanye.
Abakozi bacu mubisanzwe bari muburyo bwo "gukomeza gutera imbere no kuba indashyikirwa", kandi mugihe dukoresha ibintu byiza-byohejuru byo mu rwego rwo hejuru, agaciro keza na serivise nziza nyuma yo kugurisha, turagerageza kubona imyizerere ya buri mukiriya kuriGukuramo Acide Nucleic Acide, Uburyo bwa Magnetique, Twashyizeho uburyo bukomeye bwo kugenzura ubuziranenge.Twabonye kugaruka no guhana politiki, kandi urashobora guhana mugihe cyiminsi 7 nyuma yo kwakira wigs niba iri muri sitasiyo nshya kandi dukora serivisi yo gusana kubuntu kubisubizo byacu.Ugomba kumva udashaka kutumenyesha amakuru yandi kandi tugiye kuguha urutonde rwibiciro byapiganwa noneho.

Kits Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Iki gikoresho gikoresha inkingi ya spin hamwe na formula yakozwe nisosiyete yacu, ishobora gukuramo isuku nini kandi yujuje ubuziranenge RNA yose mumyanya yinyamanswa zitandukanye kandi ikora neza.Bitanga inkingi nziza ya ADN-isukura, ishobora gutandukanya byoroshye kandi ikanatanga ADN genomic na lysate ndengakamere na tissue, byoroshye kandi bitwara igihe;Inkingi ya RNA yonyine irashobora guhuza RNA neza kandi irashobora gutunganyirizwa icyarimwe hamwe na formula idasanzwe Ingero nyinshi.

Sisitemu yose ni RNase-Yubusa, kugirango RNA yakuweho itangirika;Buffer RW1, Buffer RW2 sisitemu yo gukaraba, kugirango RNA yabonetse idafite proteyine, ADN, ion, hamwe n’umwanda wanduye.

Ibigize ibikoresho:

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

Abakozi bacu mubisanzwe bari muburyo bwo "gukomeza gutera imbere no kuba indashyikirwa", kandi mugihe dukoresha ibintu byiza byo mu rwego rwo hejuru bifite ireme ryiza, agaciro keza na serivise nziza nyuma yo kugurisha, turagerageza kubona imyizerere ya buri mukiriya kuri Discount wholesale China Method Rna Nucleic Acide Extraction Kit hamwe nogusukura ibikoresho, Gutanga abakiriya bafite ibikoresho bikomeye nibigo, kandi akenshi biteza imbere imashini nshya nintego zubucuruzi.Dutegereje ubufatanye.
Kugabanya ibicuruzwa byinshiGukuramo Acide Nucleic Acide, Uburyo bwa Magnetique, Twashyizeho uburyo bukomeye bwo kugenzura ubuziranenge.Twabonye kugaruka no guhana politiki, kandi urashobora guhana mugihe cyiminsi 7 nyuma yo kwakira wigs niba iri muri sitasiyo nshya kandi dukora serivisi yo gusana kubuntu kubisubizo byacu.Ugomba kumva udashaka kutumenyesha amakuru yandi kandi tugiye kuguha urutonde rwibiciro byapiganwa noneho.