bitewe nubufasha buhebuje, ibicuruzwa bitandukanye byujuje ubuziranenge nibisubizo, ibiciro bikaze no gutanga neza, twishimiye gukundwa cyane mubakiriya bacu.Turi ubucuruzi bwingufu hamwe nisoko ryagutse rya 2019 igiciro cyinshi Ubushinwa Laboratoire Yumuguzi Ibikoresho byubuvuzi Laboratwari Reagents, Buri gihe dutanga ibicuruzwa byiza na serivise nziza kubantu benshi bakoresha ubucuruzi nabacuruzi.Murakaza neza cyane kwifatanya natwe, reka dushyire hamwe, kandi tuguruka inzozi.
bitewe nubufasha buhebuje, ibicuruzwa bitandukanye byujuje ubuziranenge nibisubizo, ibiciro bikaze no gutanga neza, twishimiye gukundwa cyane mubakiriya bacu.Turi ubucuruzi bwingufu hamwe nisoko ryagutse kuriUbushinwa Nucleic Acide Reagent, ADN / Rna Igikoresho cyo gukuramo, Gusa kugirango dusohoze ibicuruzwa byiza-byujuje ibyifuzo byabakiriya, ibisubizo byacu byose byagenzuwe neza mbere yo koherezwa.Buri gihe dutekereza kubibazo kuruhande rwabakiriya, kuko uratsinze, turatsinda!

Ibisobanuro

50 Imyiteguro, 200 Imyiteguro

Ibigize ibikoresho

Ibiranga ibyiza

■ Ntibikenewe ko uhangayikishwa no kwangirika kwa RNA;sisitemu yose ni RNase-Ubuntu
Kuraho neza ADN ukoresheje ADN-Isukura Inkingi
Kuraho ADN utongeyeho DNase
Ibikorwa byoroshye-byose birangirira ku bushyuhe bwicyumba
■ Kwihuta -ibikorwa birashobora kurangira muminota 30
■ Umutekano-nta reagent ukenewe
Pure Isuku ryinshi -OD260 / 280≈1.8-2.1

Porogaramu

Birakwiriye gukuramo no kweza RNA yose mubice bitandukanye byinyamanswa nshya cyangwa bikonje cyangwa ingirabuzimafatizo.

Ibipimo byibicuruzwa

Applices Porogaramu yo hasi: synthesis ya mbere ya cDNA, RT-PCR, clone ya molekile, Blot y'Amajyaruguru, nibindi.
Ingero: inyama zinyamanswa, ingirabuzimafatizo
Ingano: Tissue 10-20mg, Ingirabuzimafatizo (2-5) × 10⁶
Ubushobozi ntarengwa bwa ADN bwo guhuza inkingi yo kweza: 80 μg
Volume Ingano yo gukuraho: 50-200 μl

Urujya n'uruza rw'akazi

Igishushanyo

Amatungo Yuzuye RNA Yigunze Igikoresho cyavuwe 20mg
Icyitegererezo cyimbeba, fata 5% isukuye yose RNA 1% agar

Glycogel electrophoreis
1: Impyiko 2: Impyiko
3: Umwijima 4: Umutima

Ububiko nubuzima bwiza

Igikoresho gishobora kubikwa amezi 24 mubushyuhe bwicyumba (15-25 ℃) cyangwa 2-8 ℃ mugihe kirekire.Buffer RL1 irashobora kubikwa kuri 4 ℃ ukwezi 1 nyuma yo kongeramo merc mercaptoethanol (bidashoboka).

bitewe nubufasha buhebuje, ibicuruzwa bitandukanye byujuje ubuziranenge nibisubizo, ibiciro bikaze no gutanga neza, twishimiye gukundwa cyane mubakiriya bacu.Turi ubucuruzi bwingufu hamwe nisoko ryagutse rya 2019 igiciro cyinshi Ubushinwa Laboratoire Yumuguzi Ibikoresho byubuvuzi Laboratwari Reagents, Buri gihe dutanga ibicuruzwa byiza na serivise nziza kubantu benshi bakoresha ubucuruzi nabacuruzi.Murakaza neza cyane kwifatanya natwe, reka dushyire hamwe, kandi tuguruka inzozi.
2019 igiciro cyinshiUbushinwa Nucleic Acide Reagent, ADN / Rna Igikoresho cyo gukuramo, Gusa kugirango dusohoze ibicuruzwa byiza-byujuje ibyifuzo byabakiriya, ibisubizo byacu byose byagenzuwe neza mbere yo koherezwa.Buri gihe dutekereza kubibazo kuruhande rwabakiriya, kuko uratsinze, turatsinda!