Mwaramutse, nshuti!
Nyuma yo gukuramo aside nucleic mumyaka myinshi cyane, buriwese azi neza ko ubwiza bwo gukuramo aside nucleique arirwo rufunguzo rwo gutsinda no kumenya neza ubushakashatsi bwakurikiyeho.
Inshuro nyinshi, twita gusa niba igipimo cya OD260 / OD280 kiri murwego rwiza.Nka…
Nkuko baca umugani, ntushobora kuvuga cyane, kandi ntushobora kwishima hakiri kare.
Banza wumve Muhinduzi akubwire inkuru nto!
Imigani myiza nkiyi ikunze kuba muri laboratoire ikora aside nucleic.
Wibwiraga ko wafashe RNA gusa, Ihangane!
Mubyukuri, ni uko utazi ko Mr Protein yihishe rwihishwa.
Uratekereza ko ushaka gutuma Mr Protein igaragara hamwe na OD260 / OD280 imwe gusa?
Biracyari muto!Biroroshye cyane!
Reka dukoreshe uburyo bworoshye bwo gusiga ifeza kugirango tugufashe kubona poroteyine zihishe muri RNA!
| Ibicuruzwa byakoreshejwe | OD260 / 280 | Migikoresho |
| Foregene | 2.09 | Ubumenyi bwa ThermoTMNanoDropTMImwe Ultra-trace UV-Vis Spectrophotometer |
| Trizol | 1.91 |
Kera, kera, protein na RNA bari inshuti magara.
Mr Protein afite isoni nyinshi.
Burigihe rero burigihe RNA ikora kandi yishimye gukina nayo.
Umunsi umwe.
RNA yabwiye Bwana Protein ko igiye kwakirwa n'abandi, kandi Bwana Protein arababaje cyane.
RNA nayo yanga cyane Mr protein, maze agira ati: "Bite se ko nkwambuye, uhisha imyenda yanjye, gerageza kutabonwa nabandi, sawa?"
Bwana Protein yamwenyuye kandi arabyemera.
Ku munsi wo kurera, Bwana Protein yihishe mu myenda ya RNA ntiyaboneka.Yakurikiranye umuntu warezwe asubira murugo kandi abaho neza.
Kugereranya gukuramo RNA na F.oregeneAkagariIgiteranyoIbikoresho byo gukuramo RNA
na T ya Trizol Reagent
Turashobora kubona neza guhambira kumurongo wa 4 (Trizol), mugihe umurongo wa 3 (Foregene) usukuye.Ibisubizo byemeje ko umwanda wa proteine RNA wakuwe muburyo bwa Trizol wari ukiri ibitonyanga byinshi!Kandi umurongo wa 3 (Foregene) nta mwanda wa poroteyine uhari kuko uwagerageje yakoresheje ibikoresho byo gukuramo Foregene RNA!(Amatangazo yaje atunguranye)
Andi makuru:
https://www.foreivd.com/cell-total-rna-isolation-kit-product/
Igihe cyo kohereza: Gashyantare-10-2022
