W: Muvandimwe, inyandiko isubiza inyuma yarangiye, hanyuma intambwe yanyuma - PCR igereranya PCR!Hey, muvandimwe, PCR ni iyihe nyayo?
UMWIGISHA: PCR burigihe uzi, sibyo?
W: Urabizi, PCR nigisubizo cya polymerase, kandi ni tekinoroji yongera ADN!
UMWIGISHA: Nibyinshi… Nuburyo bwa tekiniki bwo kongera intego zikurikirana inshuro zirenga miriyoni binyuze muri primers yihariye, ariko ibi byerekana gusa ko hariho cyangwa udahari bikurikirana, kandi ntibishobora kumenya umubare wicyitegererezo cyambere.Fluorescence yuzuye PCR nuburyo bwa tekiniki bwateguwe kugirango iki kibazo gikemuke.Igihe nyacyo PCR numubare PCR (qPCR) mubisanzwe ubyerekeza.
W: Itandukaniro na PCR isanzwe iri mukigereranyo cya fluorescence?
UMWIGISHA: Yego, nibyo, murwego rwo kwagura buri cyiciro cya PCR, ubukana bwikimenyetso cya fluorescence buramenyekana, kandi umubare wambere wicyitegererezo urashobora kubarwa ukurikije ihinduka ryimbaraga za signal.
W: Birumva bigoye cyane!Ningomba kubara njyenyine?
UMWIGISHA: Ibi bigusaba kwiga buhoro, nibyiza ko ubasha kumva ihame.Noneho urashobora kwibanda kubiciro bya CT mbere, ibyo bikaba ari intangiriro yamakuru yubushakashatsi, kandi ubunini bwayo bujyanye nubunini bwikigereranyo cyambere.Hariho kandi ibishushanyo nka amplification curve hamwe no gushonga umurongo.Bafite ijambo ryanyuma niba igeragezwa ryanyu ryagenze neza cyangwa ntirishoboke.
W: Oh, muvandimwe.Noneho ngiye gukora igerageza rya qPCR!
UMWIGISHA: Witondere bidasanzwe kutongera inyandikorugero mukarere ka reagent, bitabaye ibyo kuvanga kwawe kudakoreshwa bizanduzwa byoroshye!
W: Yabonye!

Igihe nyacyo PCR Byoroshyeᵀᴹ-SYBR Icyatsi I Kit

Gukora neza, umwihariko
Kwiyunvikana cyane, guhuza neza